[1]丁淑敏,宋国强,张烨,等.去甲基蟛蜞菊内酯对香烟烟雾干预NHBE细胞损伤的保护作用[J].常州大学学报(自然科学版),2017,(04):32-38.[doi:10.3969/j.issn.2095-0411.2017.04.006]
 DING Shumin,SONG Guoqiang,ZHANG Ye,et al.Protective Effect of Demethylwedelolactone on Cigarette Smoke Extract-Induced Cytotoxicity of NHBE Cells[J].Journal of Changzhou University(Natural Science Edition),2017,(04):32-38.[doi:10.3969/j.issn.2095-0411.2017.04.006]
点击复制

去甲基蟛蜞菊内酯对香烟烟雾干预NHBE细胞损伤的保护作用()
分享到:

常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2017年04期
页码:
32-38
栏目:
生物医学工程
出版日期:
2017-07-30

文章信息/Info

Title:
Protective Effect of Demethylwedelolactone on Cigarette Smoke Extract-Induced Cytotoxicity of NHBE Cells
作者:
丁淑敏宋国强张烨李建鹏陈湘香徐德峰
常州大学 制药与生命科学学院,江苏 常州 213164
Author(s):
DING Shumin SONG Guoqiang ZHANG Ye LI Jianpeng CHEN Xiangxiang XU Defeng
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China
关键词:
去甲基蟛蜞菊内酯 香烟烟雾 肺支气管上皮细胞 超氧化物歧化酶 细胞间黏附分子-1
Keywords:
demethylwedelolactone CSE NHBE SOD ICMA-1
分类号:
R 285
DOI:
10.3969/j.issn.2095-0411.2017.04.006
文献标志码:
A
摘要:
考察去甲基蟛蜞菊内酯(Demethylwedelolactone, DWEL)对肺支气管上皮细胞(Normal Human Bronchial Epithelial,NHBE)的保护作用。以香烟烟雾提取物(Cigarette Smoke Extract,CSE)干预NHBE细胞为模型,评价DWEL对模型细胞存活率的影响。进一步采用超氧化物歧化酶(Superoxide Dismutase,SOD)和 丙二醛(Malondialdehyde,MDA)试剂盒评价DWEL对细胞氧化损伤的影响; 采用Western blot对炎症指标环氧化酶-2(Cyclooxygenase, COX-2)和细胞间黏附分子-1(Intercellular Cell Adhesion Molecule-1, ICAM-1)进行测定。结果显示,DWEL能显著提高CSE干预的NHBE细胞的存活率(70.1%),提高SOD的活力,下调MDA的含量, 使COX-2和ICMA-1的表达显著降低,并向正常方向接近。表明DWEL能降低CSE干预的NHBE损伤。
Abstract:
This study is to investigate the protection effect of demethylwedelolactone on NHBE cells. The CSE induced NHBE cells model was built up to evacuate the cell viability. SOD and MDA kit were used to evaluate the effect of DWEL on cell oxidative damage and COX-2 and ICAM-1 were measured by Western blot. The results showed that DWEL could significantly increase the survival rate of NHBE cells induced by CSE(70.1%). Pretreatment with DWEL significantly reversed the activity of SOD and the level of MDA to normal level. The protein expression levels of COX-2 and ICAM-1which related to inflammatory response were remarkablely reduced by DWEL compared with 10%CSE treatment. These results suggested that DWEL can reduce CSE-induced injury on NHBE cells

参考文献/References:

[1]高阳. 肺癌预防的研究进展[J]. 西部医学,2005,17(3):252-254.
[2]SASCO A J, SECRETAN M B, STRAIF K. Tobacco smoking and cancer: a brief review of recent epidemiological evidence[J]. Lung Cancer,2004,45:S3-S9.
[3]CHURCH D F, PRYOR W A. Free-radical chemistry of cigarette smoke and its toxicological implications[J].Environ Health Persp,1985,64:111-126.
[4]GRAY J, MAO J T, SZABO E, et al. Lung cancer chemoprevention*ACCP evidence-based clinical practice guidelines[J]. Chest,2007, 132:56-68.
[5]刘文博,谭晓斌,孙韩燕,等. 墨旱莲对香烟提取物杀伤NHBE细胞的保护作用及其机制[J]. 中国中药杂志,2012,37(16):2444-2447.
[6]LEE Y J, LIN W L, CHEN N F,et al. Demethylwedelolactone derivatives inhibit invasive growth in vitro and lung metastasis of MDA-MB-231 breast cancer cells in nude mice[J]. European Journal of Medicinal Chemistry,2012,56:361-367.
[7]BAQIR M, CHEN C Z, MARTIN R J, et al. Cigarette smoke decreases MARCO expression in macrophages:implication in myco-plasma pneumoniae infection[J]. Respir Med, 2008,102:1604-1610.
[8]HARRIS J E, THUN M J, MONDUL A M,et al. Cigarette tar yields in relation to mortality from lung cancer in the cancer prevention study II prospective cohort[J]. BMJ,2004,328:1-8.
[9]GLAUERT H P, MARTIN J B,LI J,et al. Dietary selenium fails to influence cigarette smoke-induced lung tumorigenesis in A/J mice[J]. Cancer Lett,2013,334:127-132.
[10]李冬,万海英,姚懿雯,等.香烟烟雾激活肺癌细胞Wnt/β-catenin信号途径的机制研究[J].中国癌症杂志,2014,24(12):881-889.
[11]苏志刚, 刘玉萍, 韦晔,等.香烟烟雾处理对BEAS-2B细胞FHIT基因甲基化及其mRNA表达的影响[J].毒理学杂志,2016,31(1):22-26.
[12]BAEZA-SQUIBAN A, BONVALLOT V. Airborne particles evoke an inflammatory response in human airway epithelium activation of transcription factors[J]. Cell Biology and Toxicology,1999,15:375-380.
[13]WANDA R, RANDI M,PAMELA S,et al. Gene Expression in Normal Human Bronchial Epithelial(NHBE)cells following in vitro exposure to cigarette smoke condensate[J]. Toxicological Science, 2005,86(1):84-91.
[14]PIAO M J, YOO E S, KOH Y S, et al. Antioxidant effects of the ethanol extract from flower of camellia japonica via scavenging of reactive oxygen species and induction of antioxidant enzymes[J]. International Journal of Molecular Sciences,2011,12:2618-2630.
[15]DUNINETT S, SHARMA S, HUANG M,et al. Cyclooxygenase-2 in lung cancer[J]. Prog Exp Tumor Res, 2003,37:138-162.
[16]HOSOMI Y, YOKOSE T,HIROSE Y, et a1. Increased cyelooxygenase-2(COX-2)expression occurs frequently in precursor lesions of human adenocareinoma of the lung[J]. Lung Cancer,2000,30:73-81.
[17]HUBBARD A K,ROTHLEIN R. Intercellular adhesion molecule-1(ICAM-1)expression and cell signaling cascades[J]. Free Radic Biol Med,2000,28:1379-1386.
[18]周文波,于昌松,戴宗睛,等. 粘附分子ICAM-1、VCAM-1的表达与肝细胞癌侵袭性的相关研究[J].中国现代医学杂志,2001,11(4):13-14.
[19]曾旭,罗百灵.NF-κB 及 ICAM- 1在非小细胞肺癌中的表达及其临床意义[J].中国现代医学杂志,2005,15(14):2145-2149.
[20]邵玲俐,兰晓梅,王成彬,等.葛根素对支气管上皮细胞和中性粒细胞共培养体系中黏附分子表达的影响[J].解放军医学杂志,2012,37(4):308-311.
[21]张洁,陈俊杰,陈成水,等.STAT1 和 ICAM -1在非小细胞肺癌中的表达及其临床意义[J].中国病理生理杂志,2012,28(8):1378-1382.
[22]王振冉,李扬,唐博.山姜素对肺微血管内皮细胞损伤保护机制[J].中国中西医结合杂志,2017,37(4):476-479.
[23]张振勇,曹秋婷,吴荣,等.放射性肺损伤中核因子κB和细胞间黏附分子1的表达及意义[J].中国医科大学学报,2014,43(2):155-158.

备注/Memo

备注/Memo:
收稿日期:2017-04-11。基金项目:国家自然科学基金资助项目( 81603336); 江苏省产学研前瞻项目(BY2016029-13)。作者简介:丁淑敏(1978—),女,吉林通化人,博士,讲师,主要从事天然药物有效成分研究。通讯联系人:徐德峰(1968—),E-mail: markxu@cczu.edu.cn
更新日期/Last Update: 2017-07-20