[1]刘心恺,宋厚煜,张 炯,等.定量检测幽门螺杆菌感染的胶乳比浊试剂研究[J].常州大学学报(自然科学版),2023,35(02):84-92.[doi:10.3969/j.issn.2095-0411.2023.02.010 ]
　LIU Xinkai,SONG Houyu,ZHANG Jiong,et al.Preparation of latex-enhanced immunoturbidimetric reagents for quantitative detection of helicobacter pylori infections[J].Journal of Changzhou University(Natural Science Edition),2023,35(02):84-92.[doi:10.3969/j.issn.2095-0411.2023.02.010 ]
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定量检测幽门螺杆菌感染的胶乳比浊试剂研究 ()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
第35卷
期数:
2023年02期
页码:
84-92
栏目:
生物医学工程
出版日期:
2023-03-28

文章信息/Info

Title:
Preparation of latex-enhanced immunoturbidimetric reagents for quantitative detection of helicobacter pylori infections
文章编号:
2095-0411(2023)02-0084-09
作者:
刘心恺1;  宋厚煜2;  张 炯2;  付 浩2;  赵希岳1
(1.常州大学 药学院, 江苏 常州 213164; 2.常州爱复康生物科技有限公司, 江苏 常州 213164)
Author(s):
LIU Xinkai1;  SONG Houyu2;  ZHANG Jiong2;  FU hao2;  ZHAO Xiyue1
(1.School of Pharmacy, Changzhou University, Changzhou 213164, China; 2.Changzhou Aifukang Biological Technology Co., Ltd., Changzhou 213164, China)
关键词:
幽门螺杆菌抗体检测;  胶乳增强比浊法;  试剂盒制备;  性能评价
Keywords:
helicobacter pylori antibody detection;  latex enhanced turbidimetric method;  preparation of test kit;  performance of evaluation
分类号:
R 446.62
DOI:
10.3969/j.issn.2095-0411.2023.02.010
文献标志码:
A
摘要:
将重组表达的幽门螺杆菌(Hp)上的尿素酶B(UreB)蛋白与黏附素(HpaA)蛋白通过化学反应,偶联到聚苯乙烯微球上,并置换到保存液中,制备出幽门螺杆菌的抗体检测试剂2(R2)。结合抗原抗体反应特性及降低血清中非特异性吸附,制备出幽门螺杆菌的抗体检测试剂1(R1)。R1与R2搭配使用构成了定量检测人血清中幽门螺杆菌的抗体检测胶乳试剂盒。可以发现,制备20 mL胶乳R2,包被幽门螺杆菌UreB蛋白与HpaA蛋白各1.6 mg,同时聚苯乙烯微球粒径为190 nm时,试剂效果最好。该试剂盒通过对1 000例常州本地随机临床样本与室间质评样本进行评测,发现常州本地随机临床样本的阳性率为34.6%,室间质评样本的检测结果与上海临床检验中心公布结果一致,试剂盒性能较好。
Abstract:
The urease protein and HpaA protein of the recombinant expressed Helicobacter pylori(Hp)were chemically coupled to the polystyrene microspheres and replaced in a suitable preservation solution, to prepare Helicobacter pylori antibody detection reagent 2(R2). A Helicobacter pylori antibody detection reagent 1(R1)was prepared by combining the reaction characteristics of antigen and antibody and reducing the non-specific adsorption in serum. The combination of R1 and R2 forms a latex kit for quantitative detection of Helicobacter pylori antibodies in human serum. The results showed that the best reagent was prepared by coating Helicobacter pylori urease protein and HpaA each 1.6 mg with 20 mL latex R2 and polystyrene microspheres 190 nm in diameter. The kit was tested on 1 000 random clinical samples collected in Changzhou and samples for external quality assessment. The results showed that positive rate of the random samples was 34.6%, and test results of the external quality evaluation samples were consistent with the results announced by the Shanghai Clinical Laboratory Center. As a result, the performance of the kit is good.

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相似文献/References:

[1]宋厚煜,刘心恺,张 炯,等.COVID-19抗体检测试剂盒的制备与应用[J].常州大学学报(自然科学版),2021,33(03):75.[doi:10.3969/j.issn.2095-0411.2021.03.010]
　SONG Houyu,LIU Xinkai,ZHANG Jiong,et al.Preparation and Application of COVID-19 Antibody Detection Kit[J].Journal of Changzhou University(Natural Science Edition),2021,33(02):75.[doi:10.3969/j.issn.2095-0411.2021.03.010]

备注/Memo

备注/Memo:
收稿日期: 2022-09-25。
基金项目: 苏北科技专项资助项目(SZ-LYG2017016); 常州市爱复康生物科技有限公司研发资助项目(KYH15020329)。
作者简介: 刘心恺(1995—), 男, 江苏泰州人, 硕士生。 通信联系人: 赵希岳(1965—), E-mail:xyzhao@cczu.edu.cn

更新日期/Last Update: 1900-01-01