[1]柳丽,顾亚琴,关乃富,等.雷公藤多苷对胶原诱导关节炎大鼠滑膜肥大细胞浸润和活化的影响[J].常州大学学报(自然科学版),2016,(05):82-86.[doi:10.3969/j.issn.2095-0411.2016.05.015]
　LIU Li,GU Yaqin,GUAN Naifu,et al.Effects of Tripterygium Wilfordii Polyglucosideon Infiltration and Activation of Mast Cells in Synoviumof Rats with Collagen-Induced Arthritis[J].Journal of Changzhou University(Natural Science Edition),2016,(05):82-86.[doi:10.3969/j.issn.2095-0411.2016.05.015]
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雷公藤多苷对胶原诱导关节炎大鼠滑膜肥大细胞浸润和活化的影响()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2016年05期
页码:
82-86
栏目:
生物医学工程
出版日期:
2016-09-30

文章信息/Info

Title:
Effects of Tripterygium Wilfordii Polyglucosideon Infiltration and Activation of Mast Cells in Synoviumof Rats with Collagen-Induced Arthritis
作者:
柳丽1; 顾亚琴1; 关乃富2; 王晶晶1; 付敏丽1; 周晓鹰1
1.常州大学 制药与生命科学学院,江苏 常州 213164; 2.常州市第二人民医院,江苏 常州 213003
Author(s):
LIU Li1;  GU Yaqin1;  GUAN Naifu2;  WANG Jingjing1;  FU Minli1;  ZHOU Xiaoying1
1. School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China; 2. Changzhou No.2 People’s Hospital, Changzhou 213003, China
关键词:
雷公藤多苷;  胶原诱导关节炎;  肥大细胞;  类胰蛋白酶
Keywords:
Tripterygium wilfordii polyglycoside;  collagen-induced arthritis;  mast cells;  tryptase
分类号:
R 967
DOI:
10.3969/j.issn.2095-0411.2016.05.015
文献标志码:
A
摘要:
观察雷公藤多苷(GTW)对胶原诱导关节炎(CIA)大鼠滑膜肥大细胞数量和类胰蛋白酶水平的影响。采用牛Ⅱ型胶原蛋白诱导建立CIA大鼠模型后,分设正常组、模型组和GTW组(50 mg/(kg/d),ig),正常组和模型组给予等体积溶媒,每天1次,连续28 d。监测大鼠的关节炎指数评分,HE染色评价滑膜组织病理学改变,俾士麦棕染色观察滑膜肥大细胞浸润情况,ELISA检测滑膜组织类胰蛋白酶水平。结果显示,GTW可以显著降低CIA大鼠关节肿胀度,减轻滑膜炎症和关节破坏,减少CIA大鼠滑膜肥大细胞浸润,降低滑膜组织类胰蛋白酶水平。因此,GTW可以通过减少滑膜肥大细胞浸润、抑制其活化发挥抗RA作用。
Abstract:
The study was aimed to observe the effects of Tripterygium wilfordii polyglycoside(GTW)on the number of mast cells and the level of tryptase in synovium of rats with collagen-induced arthritis(CIA). Rat arthritis was induced by bovine type-II collagen.The rats were randomly divided into normal control group, model group and GTW group, which received intragastric administration daily of dissolvant or GTW(50 mg/kg)for 28 days. The arthritis index was assessed and histological examinations were performed by hematoxylin andeosin staining.Infiltrationof synovial mast cells was determined by bismarck brown staining, and tryptase levels in synovial tissue were measured by enzyme-linked immunesorbent assay. Compared with those in model group, the macroscopic joints welling, microscopic joint erosions, synovial inflammation and mast cells infiltration were reduced significantly after GTW treatment. In addition,ELISA results showed that tryptase was decreased obviously as well. The results demonstrated that GTW effectively ameliorated synovial inflammation by inhibiting infiltration and activation of mast cells in synovium.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-01-07。基金项目:江苏省高校自然科学研究面上项目(15KJB360001)。作者简介:柳丽(1979—),女,江苏姜堰人,博士,讲师,主要从事抗炎免疫药理学研究。
更新日期/Last Update: 2016-10-10