[1]何玉财,张丹平,王利群,等.重组E. coli CCZU-K14高效合成(S)-3-羟基-4-氯丁酸乙酯研究[J].常州大学学报(自然科学版),2016,(06):1-6.[doi:10.3969/j.issn.2095-0411.2016.06.001]
 HE Yucai,ZHANG Danping,WANG Liqun,et al.Effective Synthesis of Ethyl(S)-4-Chloro-3-Hydroxybutanoate by Recombinant E. coli CCZU-K14[J].Journal of Changzhou University(Natural Science Edition),2016,(06):1-6.[doi:10.3969/j.issn.2095-0411.2016.06.001]
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重组E. coli CCZU-K14高效合成(S)-3-羟基-4-氯丁酸乙酯研究()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2016年06期
页码:
1-6
栏目:
生物医学工程
出版日期:
2016-11-30

文章信息/Info

Title:
Effective Synthesis of Ethyl(S)-4-Chloro-3-Hydroxybutanoate by Recombinant E. coli CCZU-K14
作者:
何玉财张丹平王利群卿青张跃
常州大学 制药与生命科学学院,江苏 常州 213164
Author(s):
HE Yucai ZHANG Danping WANG Liqun QING Qing ZHANG Yue
School of Pharmaceutical Engineering & Life Sciences, Changzhou University, Changzhou 213164, China
关键词:
E. coli CCZU-K14 4-氯乙酰乙酸乙酯(S)-3-羟基-4-氯丁酸乙酯 L-谷氨酰胺 β-环糊精
Keywords:
E. coli CCZU-K14 ethyl 4-chloro-3-oxobutanoate ethyl(S)-4-chloro-3-hydroxybutanoate L-glutamine β-cyclodextrin
分类号:
Q 814
DOI:
10.3969/j.issn.2095-0411.2016.06.001
文献标志码:
A
摘要:
为了避免在反应过程中加入昂贵的辅酶因子NAD+及有效地转化4-氯乙酰乙酸乙酯(COBE)合成(S)-3-羟基-4-氯丁酸乙酯((S)-CHBE),在水相反应体系中加入L-谷氨酰胺(200 mmol/L)以提高重组E. coli CCZU-K14细胞内NADH的浓度以及催化活性。当与不添加L-谷氨酰胺相比,添加L-谷氨酰胺后催化活性提高了1.1倍。进一步,在含L-谷氨酰胺(200 mmol/L)反应体系中加入了β-环糊精(n(β-环糊精):n(COBE)=0.4:1)。与不添加β-环糊精相比,添加β-环糊精后E. coli CCZU-K14催化活性提高了1.35倍。在L-谷氨酰胺-β-环糊精-水反应体系中,催化还原3 000 mmol/L COBE 反应12 h,(S)-CHBE(>99% e.e.)的产率达到94.9%。总之,研究结果为生物催化不对称高效合成(S)-CHBE工业化生产奠定了基础。
Abstract:
To avoid adding additional expensive cofactor NAD+ and effectively biotransform ethyl 4-chloro-3-oxobutanoate(COBE)into ethyl(S)-4-chloro-3-hydroxybutanoate((S)-CHBE), L-glutamine(200 mmol/L)was used to enhance the intracellular NADH content and the catalytic activity of E. coli CCZU-K14. A 1.1-fold of biocatalytic activity was increased over the control without L-glutamine. Furthermore, β-cyclodextrin(n(β-cyclodextrin):n(COBE)=0.4:1)was added into this reaction media containing L-glutamine(200 mmol/L), and the biocatalytic activity of E. coli CCZU-K14 was increased by 1.35-fold than that without β-cyclodextrin. In this β-cyclodextrin-L-glutamine-water media,(S)-CHBE(>99% e.e.)was obtained from 3 000 mmol/L COBE at 94.9% yield after 12 h. In conclusion, these results will lay the foundation for the effective industrial production of(S)-CHBE.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-06-02。基金项目:国家自然科学基金资助项目(21102011); 江苏省自然科学基金资助项目(BK20141172); 常州大学教改课题(GJY2014068)。作者简介:何玉财(1979—),男,辽宁开原人,博士,副教授,主要从事生物催化研究。
更新日期/Last Update: 2016-11-20