[1]朱梦月,雷静,周垚,等.人参二醇的微生物转化及抗HSC-T6细胞活性研究[J].常州大学学报(自然科学版),2016,(06):23-27.[doi:10.3969/j.issn.2095-0411.2016.06.005]
　ZHU Mengyue,LEI Jing,ZHOU Yao,et al.Microbial Transformation of 20(R)-Panaxadiol and Their Activity Against HSC-T6 Cells[J].Journal of Changzhou University(Natural Science Edition),2016,(06):23-27.[doi:10.3969/j.issn.2095-0411.2016.06.005]
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人参二醇的微生物转化及抗HSC-T6细胞活性研究()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2016年06期
页码:
23-27
栏目:
生物医学工程
出版日期:
2016-11-30

文章信息/Info

Title:
Microbial Transformation of 20(R)-Panaxadiol and Their Activity Against HSC-T6 Cells
作者:
朱梦月; 雷静; 周垚; 戴秋阳; 朱腊梅; 陈广通
南通大学 药学院,江苏 南通 226001
Author(s):
ZHU Mengyue;  LEI Jing;  ZHOU Yao;  DAI Qiuyang;  ZHU Lamei;  CHEN Guangtong
School of Pharmacy, Nantong University, Nantong 226001, China
关键词:
人参二醇;  微生物转化;  HSC-T6细胞;  抗肝纤维化
Keywords:
20(R)-panaxadiol;  microbial transformation;  HSC-T6;  anti-liver fibrotic
分类号:
R 284.1
DOI:
10.3969/j.issn.2095-0411.2016.06.005
文献标志码:
A
摘要:
研究了20(R)-人参二醇衍生物转化及其抗HSC-T6细胞活性。利用微生物刺囊毛霉Mucorspinosus AS 3.3450对20(R)-人参二醇进行转化,采用硅胶柱色谱、制备型高效液相色谱技术分离、纯化产物,并通过波谱数据分析确认了产物结构。采用噻唑蓝(MTT)法测试产物抗HSC-T6肝星状细胞活性。共分离鉴定出了5个化合物:3-羰基-20(R)-人参二醇(1),3-羰基-7β, 24α-二羟基-20(R)-人参二醇(2),3-羰基-7β, 24β-二羟基-20(R)-人参二醇(3),3-羰基-15α, 24α-二羟基-20(R)-人参二醇(4),3-羰基-15α, 24β-二羟基-20(R)-人参二醇(5)。MTT结果显示,化合物1～3抑制HSC-T6细胞增值的作用强于20(R)-人参二醇。
Abstract:
To investigate the derivatives of 20(R)-panaxadiol and their activities against HSC-T6 cells,20(R)-panaxadiol was treated by Mucorspinosus AS 3.3450. All products were isolated by silica gel chromatography and purified by preparative HPLC. On the basis of spectral data, structures were elucidated. The activity against HSC-T6 cells was measured by MTT method. Five biotransformation products were obtained and identified as 3-oxo-20(R)-panaxadiol(1), 3-oxo-7β, 24α-dihydroyl-20(R)-panaxadiol(2), 3-oxo-7β, 24β-dihydroyl-20(R)- panaxadiol(3), 3-oxo-15α, 24α-dihydroyl-20(R)- panaxadiol(4), 3-oxo-15α, 24β-dihydroyl-20(R)-panaxadiol(5). MTT assay revealed that compounds 1-3 showed higher inhibitory activity against HSC-T6 cells than 20(R)-panaxadiol.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-05-28。基金项目:江苏省高等学校大学生创新创业训练计划项目(201610304075Y); 南通市科技项目(MS12015044)。作者简介:朱梦月(1994—),女,江苏连云港人,本科生。通讯联系人:陈广通(1981—),E-mail:guangtong_chen@ntu.edu.cn
更新日期/Last Update: 2016-11-20