[1]赵兴青,成艳,孙秀云,等.碳酸盐矿化菌固结重金属离子Cu2+的研究[J].常州大学学报(自然科学版),2018,30(01):15-21.[doi:10.3969/j.issn.2095-0411.2018.01.003]
　ZHAO Xingqing,CHENG Yan,SUN Xiuyun,et al.Study on Heavy Metal Ion Cu2+ Mineralized by Carbonate Mineralization Bacteria[J].Journal of Changzhou University(Natural Science Edition),2018,30(01):15-21.[doi:10.3969/j.issn.2095-0411.2018.01.003]
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碳酸盐矿化菌固结重金属离子Cu2+的研究()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
第30卷
期数:
2018年01期
页码:
15-21
栏目:
环境科学与工程
出版日期:
2018-01-28

文章信息/Info

Title:
Study on Heavy Metal Ion Cu2+ Mineralized by Carbonate Mineralization Bacteria
作者:
赵兴青; 成艳; 孙秀云; 王莲莲
常州大学 环境与安全工程学院,江苏 常州 213164
Author(s):
ZHAO Xingqing;  CHENG Yan;  SUN Xiuyun;  WANG Lianlian
School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China
关键词:
碳酸盐矿化菌;  酶化作用;  微生物修复;  固结率
Keywords:
carbonate mineralization microbe;  enzymatic process;  microbial remediation;  consolidation rate
分类号:
X 15
DOI:
10.3969/j.issn.2095-0411.2018.01.003
文献标志码:
A
摘要:
选取一株从南京栖霞山矿山周边土壤中筛选出来的耐Cu菌株Cu11,对其进行了酶化实验和菌种鉴定,并利用其生长代谢过程中产生的酶化作用来分解底物尿素,产生CO2-3,与溶液中的Cu2+ 结合产生沉淀,利用EDS、XRD、SEM等分析手段对其进行了分析。结果表明:菌株Cu11是碳酸盐矿化菌,并鉴定其为Cupriavidus necator; 矿化产物主要为碳酸盐CuCO3和Cu2(OH)2CO3。对影响Cu2+ 固结率的不同因素的分析表明,随着pH的升高,Cu2+ 固结率也随之上升,当pH为13时,Cu2+ 固结率可达到97%; 接种量对Cu2+固结率的影响差异很小,维持在85%～90%; 当Cu2+ 浓度较低(0.005 mol/L和0.01 mol/L)时,Cu2+ 固结率可达到97.7%,而当Cu2+ 浓度较高(0.03～0.09 mol/L)时,Cu2+ 固结率稳定在80%左右; 随着底物浓度的增加,Cu2+ 固结率先上升后下降,在25 g/L时可达到最高值96%。
Abstract:
A Cu-tolerant strain, isolated from the soil of Mountain Qixia in Nanjing, was selected and used for enzyme digestion and identification. The was decomposited urea by enzymatic process during its growth and metabolism to produce CO2-3, and combined with Cu2+ in solution to turned to precipitation, which were analyzed using EDS, XRD, SEM and other analytical methods. The results showed that the strain Cu11 is a carbonate mineralization microbe and identified as Cupriavidus necator, the main products are CuCO3 and Cu2(OH)2CO3. The analysis of different influence on the rate of consolidation of Cu2+ showed that the Cu2+ consolidation rate increased with the increase of pH, and it reached 97% when pH was 13, the difference of inoculation amount of Cu2+ on Cu2+ consolidation rate was small, which was maintained at 85%-90%, the Cu2+ consolidation rate could reach 97.7% when the Cu2+ concentration being lower(0.005, 0.01 mol/L), while the Cu2+ concentration was high(0.03-0.09 mol/L), the Cu2+ concentration rate was only stable at about 80%, With the increase of substrate concentration, the Cu2+ consolidation rate increased first and then decreased, reaching a maximum of 96% at 25 g/L.

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备注/Memo

备注/Memo:
收稿日期:2017-04-14。
基金项目:国家自然科学基金资助项目(41302025,41541016)。
作者简介:赵兴青(1974—),女,江苏高邮人,博士,副教授。E-mail: zhaoxq@cczu.edu.cn
更新日期/Last Update: 2018-02-20