[1]杨林松,钟 慧,王璐瑶,等.怀牛膝多糖对RAW264.7细胞吞噬活性、表型和细胞因子表达的调节[J].常州大学学报(自然科学版),2020,32(04):55-62.[doi:10.3969/j.issn.2095-0411.2020.04.008]
　YANG Linsong,ZHONG Hui,WANG Luyao,et al.Regulation of Achyranthes Bidentata Polysaccharide on Phagocytic Activity, Phenotype and Cytokine Expression of RAW264.7 Cells[J].Journal of Changzhou University(Natural Science Edition),2020,32(04):55-62.[doi:10.3969/j.issn.2095-0411.2020.04.008]
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怀牛膝多糖对RAW264.7细胞吞噬活性、表型和细胞因子表达的调节()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
第32卷
期数:
2020年04期
页码:
55-62
栏目:
生物医学工程
出版日期:
2020-07-28

文章信息/Info

Title:
Regulation of Achyranthes Bidentata Polysaccharide on Phagocytic Activity, Phenotype and Cytokine Expression of RAW264.7 Cells
文章编号:
2095-0411(2020)04-0055-08
作者:
杨林松; 钟 慧; 王璐瑶; 纪蔚伟; 朱 劼; 朱孝霖
(常州大学 制药与生命科学学院,江苏 常州 213264)
Author(s):
YANG Linsong;  ZHONG Hui;  WANG Luyao;  JI Weiwei;  ZHU Jie;  ZHU Xiaolin
(School of Pharmaceutical Engineering & Life Sciences, Changzhou University, Changzhou 213164, China)
关键词:
怀牛膝多糖;  巨噬细胞;  吞噬;  表型;  细胞因子
Keywords:
Achyranthes bidentata polysaccharide;  macrophages;  phagocytosis;  phenotype;  cytokine
分类号:
R 285.5
DOI:
10.3969/j.issn.2095-0411.2020.04.008
文献标志码:
A
摘要:
研究怀牛膝多糖(ABPS)对小鼠单核巨噬细胞RAW264.7细胞活性、表型及细胞因子表达的影响。先用不同浓度的ABPS溶液(0,0.4,0.6,0.8,1.0 mg/mL)处理RAW264.7细胞48 h,后采用MTT法、中性红吞噬实验、流式细胞术分别检测了ABPS对巨噬细胞的增殖活性、吞噬能力、细胞表型及细胞因子表达的影响。ABPS对RAW264.7细胞的增殖活性没有明显的影响,但能显著提高细胞对中性红的吞噬活性和表面分子CD80,CD86及CD40的表达,降低单核细胞趋化蛋白-1(monocyte chemotactic protein 1, MCP-1)和肿瘤坏死因子α(tumor necrosis factor α, TNF-α)的表达。ABPS能够增加RAW264.7细胞的吞噬活性并促进其成熟,且能发挥一定的抗炎活性。
Abstract:
To study the effect of Achyranthes bidentata polysaccharide(ABPS)on the activity, phenotype and cytokine expression of RAW264.7 cells(murine mononuclear macrophages). RAW264.7 cells were treated with different concentrations of ABPS solution(0, 0.4, 0.6, 0.8, 1.0 mg/mL)for 48 hours. Then, the effects of ABPS on the proliferation, phagocytosis, cell phenotype and cytokine expression of macrophage were analyzed by MTT assay, neutral red phagocytosis assay and flow cytometry respectively. ABPS had no significant effect on the proliferation of RAW264.7 cells, but significantly increased cell phagocytic activity and the expressions of CD80, CD86 and CD40. In addition, ABPS decreased the expressions of monocyte chemotactic protein 1(MCP-1)and tumor necrosis factor α(TNF-α). ABPS increased the phagocytic activity of RAW264.7 cells, promoted cell maturation, and exerted certain anti-inflammatory activity.

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备注/Memo

备注/Memo:
收稿日期:2020-01-28。
基金项目:国家自然科学基金资助项目(21676029)。
作者简介:杨林松(1974—),女,河南南阳人,博士,副教授。E-mail: linsongyang@cczu.edu.cn
引用本文:杨林松,钟慧,王璐瑶,等. 怀牛膝多糖对RAW264.7细胞吞噬活性、表型和细胞因子表达的调节[J]. 常州大学学报(自然科学版),2020,32(4):55-62.
更新日期/Last Update: 2020-07-30