[1]黄杰,高广达,朱军,等.反胶团萃取α－淀粉酶的优化实验[J].常州大学学报(自然科学版),1994,(01):5-9.
点击复制

反胶团萃取α－淀粉酶的优化实验
分享到：

常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
1994年01期
页码:
5-9
栏目:
出版日期:
1900-01-01

文章信息/Info

Title:
The Extraction of α-Amylase by Using DMBAC /Heptane Reversed Micelles System
作者:
黄杰; 高广达; 朱军; 吴冀川
关键词:
?amylaze;  reversed micellar;  extraction
Keywords:
?amylaze reversed micellar extraction
分类号:
O652.62
摘要:
采用新型ＤＭＢＡＣ／正庚烷反胶团体系，进行萃取分离提纯α－淀粉酶．考察了表面活性剂浓度、ｐＨ、离子强度、温度等参数对萃取的影响，确定了优化工艺条件，单级萃取α－淀粉酶的总收率可达７８．０％．
Abstract:
The extraction of α-amylaxe by using a reversed micellar phase to transport the enzyme from one aqueous phase to another, has been studied. As a special cationic surfactant DMBAC was used to solutilize α-amylase in heptane and the effects of pH,ionic strength,temperature on the extraction performace and the specific activity of enzyme have also investigated.

备注/Memo

备注/Memo:
江苏省科委应用基础研究基金
更新日期/Last Update: 1900-01-01