[1]李慧蓉,尹　艳,高云霞.聚乙烯醇包埋固定黄孢原毛平革菌方法的探讨[J].常州大学学报(自然科学版),2003,(02):5-8.
　LI Hui -rong,YIN Yan,GAO Yun -xia.Probing of the Entrapment Method for Phanerochaete chrysospor ium with PVA[J].Journal of Changzhou University(Natural Science Edition),2003,(02):5-8.
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聚乙烯醇包埋固定黄孢原毛平革菌方法的探讨()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2003年02期
页码:
5-8
栏目:
出版日期:
2003-06-25

文章信息/Info

Title:
Probing of the Entrapment Method for Phanerochaete chrysospor ium with PVA
作者:
李慧蓉1 ;  尹　艳2 ;  高云霞2
1.江苏工业学院环境与安全工程系, 江苏 常州 213016
Author(s):
LI Hui -rong 1 ;  YIN Yan 2 ;  GAO Yun -xia 2
1 .Department of Envi ronmental and Safety Eng ineering , Jiangsu Poly technic University , Changzhou 213016 , China
关键词:
黄孢原毛平革菌; 聚乙烯醇; 包埋; 比布列希猩红; 脱色降解
Keywords:
Phanerochaete chrysosporium ; PVA ; ent rapment ; Biebrich Scarlet ; decolorization and degradation
分类号:
Q 93
文献标志码:
A
摘要:
以PVA 为包埋剂对黄孢原毛平革菌进行固定, 并建立包埋颗粒对染料比布列希猩红的反应体系。表明:PVA 浓度为 10 %较适合;交联剂H3BO3 的pH 为6.7 有利于成球;海藻酸钠的加入可防止粘连发生;复合包埋、冷冻法、Al2 (SO4)3 处理 小球或在培养基中添加CaCO3 等, 均能或提高小球的机械强度或促进降解反应, 但有时也会引起负面效应。
Abstract:
Phanerochaete chrysosporium w as entrapped with PVA and the reaction system for dye Biebrich Scarlet w ith entrapment particles was set up .The study showed :the appropriate concent ration of PVA was 10 %;pH6.7 of crosslinking agent H3BO3 was of benefit to the forming of beads ;the addi tion of sodium alginate could prevent agglomeration ;the complex -supports -entrapment , the f reezing mehthod , the treatment of beads w ith Al2 (SO4)3 or the supplemnt of CaCO3 in culture medium ect .could increase the strength of beads o r promote the deg rading of dye , but it was possible that they also caused some negative effects .

参考文献/References:

[1] 李峰, 吕锡武, 严伟.聚乙烯醇作为固定化包埋剂的研究[J] .中国给排水, 2000, 16 (12):14 -16.
[2] 李学梅, 林建平, 岑沛霖.霉菌固定化综述[J] .生物工程 进展, 1999 , 19 (4):62 -66.
[3] 李慧蓉, 陈建海.黄孢原毛平革菌对五氯苯酚生物降解研究 [J] .江苏石油化工学院学报, 1999 , 11 (2):24 -27.
[4] 李慧蓉.真菌降解技术中载体固定和表面活性剂的作用[J] . 上海环境科学, 2000 , 19 (5):235 -239.
[5] Pasz czynski A , C raw f ord R L.Pot ential for Bioremediation of Xenobiotic Compounds by the Whit e -rot Fungus Pha nerochaete chrysosporium [J] .Biotechnol Prog , 1995 , 11 (4):368 - 379.
[6] Wang X , Ruckenst ein E . Immobi lization of Pha nerochaete chrysosporium on Porus Polyurethane Particles with Application t o Biodegradation of 2 -chlorophenol [J] .Biotech Techniq, 1994 , 8 (5):339 -344.
[7] Linko Y -Y, Leisola M , Lindholm N , et al.C ont inuous Production of Lignin Peroxidase by Phanerochaet e chrysosporium [J] .Journ Bi ot echnol , 1986 , 4 , 283 -291.
[8] Yusef H H , Ghanem K M , El -Kassas H Y .Optimization of Biot reatment of Paper Industrial Eff luent w ith Pha nerochaete chrysosporium [J] .Fresenius Envir Bull , 1999 , 8 , 668 -677.

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备注/Memo

备注/Memo:
基金项目:江苏省高校自然科学基金资助(02KJB610002) 作者简介:李慧蓉(1947 -), 女, 上海人, 教授, 主要从事环境生物技术的研究;2 -本院2001 届环境与安全工程 系毕业生。
更新日期/Last Update: 2003-06-25