白腐真菌锰过氧化物酶的研究-常州大学学报(自然科学版)

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作者:: 陈兆林 ¹ ; 胡大烽 ² ; 李慧蓉 ¹; 1.江苏工业学院环境与安全工程系, 江苏常州?? 213016????2.中国建筑装饰研究院, 北京?? 100023?? ?? ?? ?? ????

Author(s):: CHEN Zhao-lin¹ ; HU Da-feng² ; LI Hui-rong¹; Department of Environmental and Safety Engineering, Jiangsu Polytechnic University, Changzhou 213016, China; 2. Building & Decoration Design and Research Institute of China, Beijing 100023

Abstract:: By estabishing liquid systems of white rot fungi such as Phanerochaete chrysosporium, Trametes versicolor, Trametes hirsuta, Trametes hispida, the effects of o.everal factors on the produc? tion of Manganese Peroxidase were studied. The investigation showed: ①Mn2+ was necessary for produc? tion of Manganese Peroxidase to all tested species; ② VA ( 0. 4 mmol/L), Tween80 ( 0. 1%) and oxalic acid (5 mmol/L) could stimulate Manganese Peroxidase synthesis to all tested species; ③to Phanero? chaete chrysosporium, higher nitrogen inhibited activities of Manganese Peroxidase; to Trametes hispida, amount of nitrogen had no effect on Manganese Peroxidase synthesis; ④L-Phenylalanine (20 mmol/L), D-Phenylalanine (20 mmol/L) and Dithiothreitol (0. 1%) supressed Manganese Peroxidase synthesis; ⑤the ability of Manganese Peroxidase production by Trametes versicolor was higher than that of Phanero? chaete chrysosporium, and the duration time of Manganese Peroxidase production by Trametes versicolor was longer.