[1]董　艳,邱岳进,孙贤祥.盐酸氢氯噻嗪分子印迹聚合物的实验研究[J].常州大学学报(自然科学版),2007,(04):21-24.
　DONG Yan,QIU Yue -jin,SUN Xian -xiang.Experimental Investigation on the Molecularly Imprinted Polymer Receptor for Hydrochlorothiazide[J].Journal of Changzhou University(Natural Science Edition),2007,(04):21-24.
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盐酸氢氯噻嗪分子印迹聚合物的实验研究()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2007年04期
页码:
21-24
栏目:
出版日期:
2007-12-25

文章信息/Info

Title:
Experimental Investigation on the Molecularly Imprinted Polymer Receptor for Hydrochlorothiazide
作者:
董　艳1 ;  邱岳进2 ;  孙贤祥3
1.常州市华生制药有限公司质量管理部, 江苏 常州 213022 ;2.常州出入境检验检疫局;3.江苏工业学院
Author(s):
DONG Yan1 ;  QIU Yue -jin2 ;  SUN Xian -xiang3
1 .Department of Quali ty &Manag ement , Hua Sheng Drug &Pharmaceutical limited Company , Changzho u 213022 , China ;2 .Chang zhou Bureau of Commo dity Inspectio n &Quarant ine on Entering and Exiting ;3 .Jiang su Poly technic Universi ty
关键词:
盐酸氢氯噻嗪; 分子印迹聚合物; 印迹条件
Keywords:
Hy dro chloro thiazide ; molecularly imprinted polymer ; imprinting condi tion
分类号:
Q 632 ;R 971
文献标志码:
A
摘要:
以盐酸氢氯噻嗪为模板分子, 以甲基丙烯酸甲酯(MAA) 为功能单体, 以三-羟甲基丙烷-三-丙烯酸酯为交联剂, 偶氮二异丁腈为引发剂, 并通过实验条件的优选, 合成了对氢氯噻嗪具有特异作用的分子印迹聚合物。通过测定印迹聚合物的 结合量, 评价了其对氢氯噻嗪的亲和力。S catchard 分析表明, 在印迹聚合物中存在对氢氯噻嗪形成不同亲合力的两类结合位 点, 这两类不同结合位点的离解常数分别为K d1 =57.0 μmol/ L 和K d2 =3.96 m mol/ L ;其最大表观结合量分别为Qmax 1 = 5.59 μm ol/ g 和Qmax 2 =216.4 μmol/ g 。
Abstract:
A synthetic polymer selective to Hydro chloro thiazide w as prepared by a non -covalent ly imprinted technique , using Hydrochlorothiazide as a template , methyl methacrylate as a functio nalmonomer and t ri - [ hy droxymethy l] -propan -tri -acrylic acid as a cross -linker , and methanol as a porog enic solvent .After extraction of template , affinity of the polymer imprinted with Hydro chloro thiazide w as examined in re-binding experiment .From the Scatchard analysis , binding site heterog eneity is obvious and two types of binding sites can be ident ified .The equilibrium dissociation co nstant K d1 , the apparent maximum amount Qmax1 and the binding constant Ka1 of the hig her aff inity binding sites can be calculated to be 57.0 μmo l/L , 5.59 μmo l/g , and 1.75 ×104 (mol/L)-1 ;w hile K d2 , Qmax2 , and K a2 of the lower affinity binding sites were calculated to be 3.96 mmol/L , 216.4 μmol/g and 2.53 ×102 (mo l/L)-1 , respectively .

参考文献/References:

[1] Anders son L I .Malecu lar imp rin tin g :devel opmen ts and applicat ions in the anal yti cal chemist ry f ield [J] .J Ch romatogr B , 2000 , 745 :3 -13 .
[2] Haupt K , M osbach K .Molecularly imp rint ed polymers and th eir use in bi omimet ric Sens ors [J] .Chem Rev , 2000 , 100 : 2 495 -2 504.
[3] M osque N , Marce R M , Borrull F.M olecu larly imp rint ed polymers :new t ail or -made mat erials f or select ive solid - phase ex t raction [J] .Trends in Anal Ch em , 2001 , 20 :477 -485.
[4] Turk ew it sch P, Wandel t B, Darling G D , et al.Flu orescent fun cti onal recogni tion sit es through molecular imprint ing .A polymer -b ased f luorescent chem osen sornfo r aqueous cAMP [J] .Anal Ch em , 1998 , 70 :2 028 -2 030 .
[5] 郭洪声, 何锡文, 周杰, 等.药物氟哌酸分子模版聚合物的分 子识别特性[J] .分析化学, 2001 , 29 :128 -132 .

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　SU N Xian- x iang,Q IU Yue- jin,XIE Ai- juan,et al.Synthetic Receptor for Propranolol- Synthesis and Molecular Recognition of Molecularly Imprinted Polymer[J].Journal of Changzhou University(Natural Science Edition),2008,(04):50.

备注/Memo

备注/Memo:
作者简介:董艳(1973 -), 女, 江苏徐州人, 助理工程师;通讯联系人:孙贤祥。
更新日期/Last Update: 2007-12-25