[1]冯胜,李定龙,高?? 光.淡水湖泊沉积物微生物多样性研究方法进展[J].常州大学学报(自然科学版),2010,(02):66-72.
 FENG Sheng,LI Ding- long,GAO Guang.Research Progress on Microbial Community Structure and Diversity in the Sediment of Freshwater Lakes[J].Journal of Changzhou University(Natural Science Edition),2010,(02):66-72.
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淡水湖泊沉积物微生物多样性研究方法进展()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2010年02期
页码:
66-72
栏目:
出版日期:
2010-06-25

文章信息/Info

Title:
Research Progress on Microbial Community Structure and Diversity in the Sediment of Freshwater Lakes
作者:
冯胜1 李定龙1 高?? 光2
1常州大学环境与安全工程学院, 江苏 常州 213016; 2?? 中国科学院南京地理与湖泊研究所, 江苏 南京 210008
Author(s):
FENG Sheng1 LI Ding- long1 GAO Guang 2
1. School of Env ironmental and Safety Eng ineer ing, Chang zhou University, Chang zhou 213164, Ch-i na; 2. Nanjing Institute of Geog raphy and Limno logy, Chinese Academy of Sciences, N anjing 210008, China
关键词:
湖泊沉积物 微生物多样性 研究方法
Keywords:
fr eshw ater sediment micr obial community analysis resear ch method
分类号:
X 172
文献标志码:
A
摘要:
湖泊生境中营养的循环主要受到表层沉积物中微生物代谢活动的影响, 加强对该生境中微生物群落结构和多样性的研 究, 对于阐明微生物群落与其生境的关系, 揭示群落结构与功能的联系, 进而指导微生物群落功能的定向调控。简要介绍了传 统的分离培养方法和基于微生物生理生化分析的生物标记方法及以16S rDNA ( RNA) 为目标物的现代分子生物学技术在湖泊 沉积物中微生物群落结构及多样性研究中的应用; 指出了现代分子生物学方法结合传统的微生物研究手段将为湖泊沉积物微生 物多样性研究开辟新的道路。
Abstract:
Nutrient cycles in f reshwater lakes are largely inf luenced by micro be metabo lic processes lo cated in the sur face lay er s o f sediment s. Study o n micro bial community st ructur e and div ersity in this habitat is impo rtant for exploit ing microbe resources- clarifying the relat ion o f micro bial community and it s hab-i tat s, disclo sing the connect ion betw een the community st ructure and it s funct ion, and then co nsequently guiding direct ional adjustment to contr ol of microbial community funct io n. The aim of this review is to interpret recent studies in w hich molecular methods w ere used to analyze microbe community in lake sed-i ment s. In the past , know ledg e abo ut community st ructure and div ersity of env ir onmental microo rganisms based on t radit ional culturing and iso lat ing technolog y, then biomarker metho ds w ere applied, w hich is based on the chemical component analy sis of microbe, and modern molecule bio logy techno logies based on 16SrDNA f ing erprint analysis, w hich can accurately disclose the species diversity and inher itance div ersity of microorg anisms in natural habit s and prov ide us w ith external info rmat ion about micro bial community st ructure. Point out ex perimental st rateg ies based on the combinat ion o f mo lecular techniques w ith t rad-i t ional cultivat ion- dependent methods have g reat potent ial in revealing some of the hidden complex ity of natur al microbial ecosystems.

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备注/Memo

备注/Memo:
基金项目: 国家科技重大专项( 2009ZX07101- 013) ; 中国科学院可再生能源与天然气水合物重点实验室资助 ( 0907k6) ; 常州大学科技基金( ZMF09020013) 作者简介: 冯胜( 1975- ) , 男, 河南南阳人, 博生。
更新日期/Last Update: 2010-06-25