[1]王利群,孙晓慧,曹利民,等.发酵液中透明质酸含量检测方法的优化与比较[J].常州大学学报(自然科学版),2011,(01):43-47.
　WANG Li-qun,SUN Xiao-hui,CAO Li-min,et al.Comparison and Optimization of Two Quatitative Assays of Hyaluronic Acid in Fermentation Broth[J].Journal of Changzhou University(Natural Science Edition),2011,(01):43-47.
点击复制

发酵液中透明质酸含量检测方法的优化与比较()
分享到：

常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2011年01期
页码:
43-47
栏目:
环境科学与工程
出版日期:
2011-01-01

文章信息/Info

Title:
Comparison and Optimization of Two Quatitative Assays of Hyaluronic Acid in Fermentation Broth
作者:
王利群1; 孙晓慧1; 曹利民2; 陈萦慈1; 赵 婕1
1.常州大学 制药与生命科学学院,江苏 常州 213164; 2.常州方圆制药有限公司,江苏 常州 213000
Author(s):
WANG Li-qun1; SUN Xiao-hui1; CAO Li-min2; CHEN Ying-ci1; ZHAO Jie1
1.School of Pharmaceutical Engineering and Life Science, Changzhou University,Changzhou 213164,China; 2.Changzhou Fangyuan Pharmaceutical Co., Ltd., Changzhou 213000,China
关键词:
透明质酸;  含量检测;  CTAB比浊法;  Bitter-Muir法;  发酵液
Keywords:
hyaluronic acid;  quantitative analysis;  CTAB turbidimetric method;  Bitter-Muir method;  fermentation broth
分类号:
Q 503
文献标志码:
A
摘要:
对透明质酸含量检测方法CTAB比浊法和Bitter-Muir法进行了优化,并用于发酵液中透明质酸含量的检测。优化结果为:CTAB比浊法最佳检测波长为400 nm,CTAB溶液质量浓度为5 g/L,反应温度为室温,络合时间为3 min,回归线性方程y=0.007 1x-0.101 8,R=0.999 9,发酵液中加标平均回收率101.29%,平均RSD值为0.70%; Bitter-Muir法最佳检测波长为540 nm,水浴时间为10 min,线性回归方程y=0.005 8-0.199 3x,R=0.996 5,发酵液中加标平均回收率83.87%,平均RSD值为2.70%。结果表明,用于发酵液中透明质酸含量的检测,CTAB比浊法更准确、更精确、更高效。
Abstract:
The Bitter-Muir method and CTAB turbidimetric method were optimized to assay the concentration of hyaluronic acid in fermentation broth.The optimum conditions of CTAB method are wavelength 400 nm, 5 g/L CTAB, room temperature, and reaction time 3 min. The regression equation is y=0.007 1x-0.101 8, R=0.999 9. The precision and recovery of this method are within 0.70% and 101.29%, respectively. The optimum conditions of Bitter-Muir method are wavelength 540 nm, reaction time 10 min. The regression equation is y=0.005 8-0.199 3x, R=0.9965. The precision and recovery of this method are within 2.70% and 83.87%, respectively. The results showed that CTAB method is superior to Bitter-Muir methodin terms of accuracy, precision and efficiency.

参考文献/References:

[1]凌沛学,贺艳丽,郭学平,等.透明质酸[M].北京:中国轻工业出版社,2007.
[2]生举正,凌沛学,王凤山.透明质酸生物合成的研究进展[J].中国生化药物杂志,2009,30(2):135-138.
[3]刘丽,王强,陈永浩.微生物发酵生产透明质酸研究进展[J].食品与发酵,2009,35(2):125-128.
[4]Yu H M, Stephanopoulos G. Metabolic engineering of Escherichia coli for biosynthesis of hyaluronic acid[J]. Metab Eng, 2008, 10:24-32.
[5]Bitter T, Muri H M. A modified uronic acid carbazole reaction[J]. Anal Biochem, 1962,4:330-334.
[6]文程,于慧敏,孙云鹏,等.高效测定发酵液中透明质酸含量的改良CTAB浊度法[J].中国生物工程杂志,2010,30(2):89-93.
[7]陈永浩,王强. 发酵液中透明质酸含量快速检测方法研究[J].食品与发酵工业,2009,35(1):135-139.
[8]刘屹然,陈林海,李宗伟,等. 发酵液中透明质酸含量测定方法的比较研究[J].河南师范大学学报,2006,34(1):102-105.
[9]Chong B F,Nielsen L K. Amplifying the cellular reduction potential of Streptococcus zooepidemicus[J]. Biotechnol, 2003, 100:33-34.

相似文献/References:

[1]周维杰,席海涛,孙小强.含荧光基团透明质酸衍生物的合成及其荧光性能[J].常州大学学报(自然科学版),2013,(04):57.[doi:10.3969/j.issn.2095-0411.2013.04.012]
　ZHOU Wei jie,XI Hai tao,SUN Xiao qiang.Synthesis and Fluorescence Properties of Hyaluronic Acid Derivatives Containing Fluorescent Groups[J].Journal of Changzhou University(Natural Science Edition),2013,(01):57.[doi:10.3969/j.issn.2095-0411.2013.04.012]
[2]黄晓佳,生程钜,周俊涛,等.含奎宁水凝胶的构建及其抗炎作用[J].常州大学学报(自然科学版),2020,32(05):81.[doi:10.3969/j.issn.2095-0411.2020.05.011]
　HUANG Xiaojia,SHENG Chengju,ZHOU Juntao,et al.Preparation of Hydrogel with Quinine and Its Anti-Inflammatory Effects[J].Journal of Changzhou University(Natural Science Edition),2020,32(01):81.[doi:10.3969/j.issn.2095-0411.2020.05.011]

备注/Memo

备注/Memo:
作者简介:王利群(1972-),女,江苏常州人,副教授。
更新日期/Last Update: 2011-01-01