[1]秦 薇,梁玉婷,刘勇俊,等.石油污染土壤中降解菌的分离鉴定及降解基因筛选[J].常州大学学报(自然科学版),2012,(04):1-7.
　QIN Wei,LIANG Yu-ting,LIU Yong-jun,et al.Isolation,Identification and Gene Cloning of Oil-Degrading Bacteria in Contaminated Soil[J].Journal of Changzhou University(Natural Science Edition),2012,(04):1-7.
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石油污染土壤中降解菌的分离鉴定及降解基因筛选()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2012年04期
页码:
1-7
栏目:
环境科学与工程
出版日期:
2012-09-30

文章信息/Info

Title:
Isolation,Identification and Gene Cloning of Oil-Degrading Bacteria in Contaminated Soil
作者:
秦 薇; 梁玉婷; 刘勇俊; 刘雨佳; 赵 远
常州大学 环境与安全工程学院,江苏 常州 213164
Author(s):
QIN Wei; LIANG Yu-ting; LIU Yong-jun; LIU Yu-jia;  ZHAO Yuan
School of Environmental and Safety Engineering,Changzhou University,Changzhou 213164,China
关键词:
石油烃降解菌;  分离鉴定;  生物降解;  基因
Keywords:
petroleum hydrocarbon degradation bacterium;  isolation and identification;  biodegradation;  gene
分类号:
X 172
文献标志码:
A
摘要:
为了得到高效的石油降解菌,以原油为唯一碳源配制培养基,从金南油田油污染土壤中选取4处样品富集培养,纯化出14株细菌,8株放线菌,9株真菌; 通过生理生化反应以及16S rDNA鉴定,确定了C-1和H-1菌株均为芽孢杆菌属; 通过降解性能实验和优化实验,初步绘制了C-1和H-1的生长曲线并确定了最佳生长条件和降解率; 经查询相关文献,设计了6对降解基因引物,应用PCR的方法对所筛选出的降解菌进行基因克隆,确定C-1与H-1菌中含有可降解芳香族化合物的谷胱甘肽S-转移酶(GSTs)的基因。
Abstract:
In order to get high degradation of oil bacteria,this study used oil as the only carbon source, chose samples from four places in the soil polluted by oil of Jinna oil field and purified the 14 strains of bacteria,8 strains of actinomyces,and 9 strains of fungi.Through the physiological andbiochemical reaction and identification of 16S rDNA,the C-1 and H-1 strains were determined to belong to the genus bacillus.After degrading performance and optimization experiments,C-1 and H-1 growth curves were drawn and the best growthconditions and degradation rate were identified.Based on the design of 6 pairsof primers of degradation genes and the PCR method for cloning genes after referring to the relevant references,C-1 and H-1 bacteria were found out to containgenes of glutathione S-shift enzyme(GSTs)that can degrade biodegradable aromatic compounds.

参考文献/References:

[1]李宝明.石油污染土壤微生物修复的研究[D].北京:中国农业科学院,2007.
[2]陆秀君,郭书海,孙清,等.石油污染土壤的修复技术研究现状及展望[J].沈阳农业大学学报,2003,34(1):63-67.
[3]Tano Debrah K,Fukuyama S,Otonari N,et al.An Inoculum for the Aerobic Treatment of Wastewaters with High Concentrations of Fats and Oils[J]. Bioresource Technology,1999,69(2):133-139.
[4]Van Hamme J D, Ward O P.Physical and meta-bolic interactions of Pseudomonas sp.strain JA5- B45 and Rhodococcus sp.strain F9-D79 during grow them crudeoil and effect of a chemical surfactant on them[J].App l Environ Microbiol,2001,67(10):4874-4879.
[5]Deborah D R,Joann A M,Cerniglia C E.Utilization of mix tures of polycyclic aromatic hydrocarbons by bacteria isolated from contaminated sediment[J].FEMSM icrobiol Eco,2002,41(1):1-7.
[6]梁生康,王修林,汪卫东,等.高效石油降解菌筛选及其在油田废水深度处理中的应用[J].化工环保,2004,24(1):41-45.
[7]袁红莉,杨金水,王占生,等.降解石油微生物菌种的筛选及降解特性[J].中国环境科学,2003,23(2):157-161.
[8]张景来,李正要,汪莉,等.海水中原油的生物降解[J].北京科技大学学报,2003,25( 5 ):410-413.
[9]Whyte L G,Bourbonnier E L,Greer C W.Biodegradation of petroleum hydrocarbons by psychro-trophic Pseudomonas strains possessing both alkane(alk)and naphthalene(nah)catabolic pathways[J].Appl Environ Microbiol,1997,63(9):3719-3723.
[10]Folsom B R,Schieche D R,Digrazia P M,et al.Microbial desulfurizationof a lkylated dibenzothio-phenes from a hydrodesulfurized middle distillate by Rhodococcus erythropolis 1-19[J].Appl Environ Microbiol,1999,65(11):4967-4972.
[11]Koike K,Ara K,Adach I S,et al.Regiospecific internal desaturation of aliphatic compounds by a mutant Rhodococcus Strain[J].Appl Environ Microbiol,1999,65(12):5636-5638.
[12]徐金兰,黄廷林,唐智新,等.高效石油降解菌的筛选及石油污染土壤生物修复特性的研究[J].环境科学学报,2007,7(4):62-67.
[13]张鲁进,杨谦,陈中祥,等.两株石油降解菌的降解性能研究[J].南京理工大学学报,2010 34(6):850-854.
[14]Juarez M J B,Zafra-Gamez A,Luzon-Toro B,et a1.Gas chromatographic-massspectrometric study of the degradation of phenolic compounds in wastewater olive oil by azotobacter chroococcum[J].Bioresource Technology, 2008,99(7):2392-2398.
[15]中国石化总公司环境监测总站.GB/T16488-1996 红外分光光度法[S].北京:中国标准出版社,1996.
[16]郝士海.现代细菌学培养基和生化试验手册[M].北京:中国科学技术出版社,1992:103-110.
[17]东秀珠,蔡妙英.常见细菌鉴定手册[M].北京:科学出版社,2001.
[18]李友发.福建稻田细菌群落PCR-DGGE分析和石油烃降解基因克隆[D].福州:福建农林大学,2008.
[19]Stairs T H M,Rsthlisberger M,Witholt B,et a1.Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains[J].EnvironMicrobiol,1999,1(4):307-317.
[20]赵晴.疏水石油烃降解菌强化降解系统的构建及其降解能力的研究[D].武汉:武汉大学,2005.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(41101233); 常州大学博士基金项目(ZMF1002125) 作者简介:秦薇(1989-),女,江苏南通人,硕士生; 通讯联系人:赵远。
更新日期/Last Update: 2012-09-30