[1]堵燕钰,罗漪涟,吴冬梅,等.在线净化/LC-MS-MS测定淡水鱼中孔雀石绿及隐性孔雀石绿[J].常州大学学报(自然科学版),2015,(01):71-75.[doi:10.3969/ j.issn.2095-0411.2015.01.014]
　DU Yan-yu,LUO Yi-lian,WU Dong-mei,et al.Determination of Malachite Green and Leucomalachite Green in Freshwater Fish by Online Purification and LC-MS-MS[J].Journal of Changzhou University(Natural Science Edition),2015,(01):71-75.[doi:10.3969/ j.issn.2095-0411.2015.01.014]
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在线净化/LC-MS-MS测定淡水鱼中孔雀石绿及隐性孔雀石绿()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2015年01期
页码:
71-75
栏目:
化学化工
出版日期:
2015-01-25

文章信息/Info

Title:
Determination of Malachite Green and Leucomalachite Green in Freshwater Fish by Online Purification and LC-MS-MS
作者:
堵燕钰; 罗漪涟; 吴冬梅; 王博超; 王洁琼
常州市农畜水产品质量监督检验测试中心,江苏 常州 213002
Author(s):
DU Yan-yu; LUO Yi-lian; WU Dong-mei; WANG Bo-chao; WANG Jie-qiong
Agriculture Beast and Fishing Product Quality Supervision Inspection and Test Center of Changzhou,Changzhou 213002,China
关键词:
在线净化;  LC-MS-MS;  孔雀石绿;  测定
Keywords:
online purification;  LC-MS-MS;  malachite green;  determination
分类号:
O 657.6
DOI:
10.3969/ j.issn.2095-0411.2015.01.014
文献标志码:
A
摘要:
研究了一种在线净化/LC-MS-MS联用技术测定淡水鱼中孔雀石绿(MG)及隐性孔雀石绿(LMG)残留的方法。样品加内标,经乙腈涡旋提取、离心,提取液经在线净化柱(Cyclone P)净化,以10 mmol/L乙酸铵-乙腈溶液将目标物洗脱至C18柱上,用LC-MS-MS检测。方法在0.1～10 μg/kg质量浓度范围内有良好的线性关系,MG、LMG的检出限为:0.1 μg/kg,3个浓度加标回收率为84.5%～99.6%,相对标准偏差为2.7%～5.3%。该方法前处理简便快速、试剂用量少、回收率高、灵敏度和重现性好,适用于水产品中孔雀石绿及其代谢物残留量分析。
Abstract:
A method was established for determination of malachite green(MG)and leucomalachite green(LMG)residues in freshwater fish by online purification and LC-MS-MS technology. The samples were vortex extracted and centrifuged with acetonitrile after adding internal standard. The extract was purifiedby online column(Cyclone P), and then the target compound was eluted to C18 column with 10 mmol/L ammonium acetate-acetonitrile solution for further detection by LC-MS-MS. The method has a good linearity concentration range from 0.1 to 10 μg/kg, and the detection limit of MG/LMG is 0.1 μg/kg. Therecoveries are 84.5%-99.6% by adding 3 levels of internal standard and the relative standard deviation values are 2.7%-5.3%. The method is simple and rapid,reagent-saving and with high recoveries, sensitivity and good reproducibility,therefore it is suitable for malachite green(MG)and leucomalachite green(LMG)residues analysis.

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备注/Memo

备注/Memo:
作者简介:堵燕钰(1982-),女,江苏常州人,农艺师,主要从事农畜水产品质量安全检测方面研究。
更新日期/Last Update: 2015-01-25