[1]刘文杰,杜涛,王亮,等.新型光敏性表面活性剂对BSA的光控构象研究[J].常州大学学报(自然科学版),2016,(05):28-33.[doi:10.3969/j.issn.2095-0411.2016.05.005]
　LIU Wenjie,DU Tao,WANG Liang,et al.The Optically Controlled Conformational Studies of BSA with Novel Photosensitive Surfactants[J].Journal of Changzhou University(Natural Science Edition),2016,(05):28-33.[doi:10.3969/j.issn.2095-0411.2016.05.005]
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新型光敏性表面活性剂对BSA的光控构象研究()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2016年05期
页码:
28-33
栏目:
化学化工
出版日期:
2016-09-30

文章信息/Info

Title:
The Optically Controlled Conformational Studies of BSA with Novel Photosensitive Surfactants
作者:
刘文杰; 杜涛; 王亮; 袁华; 左士祥; 姚超
常州大学 石油化工学院,江苏 常州 213164
Author(s):
LIU Wenjie;  DU Tao;  WANG Liang;  YUAN Hua;  ZUO Shixiang;  YAO Chao
School of Petrochemical Engineering,Changzhou University, Changzhou 213164, China
关键词:
光敏表面活性剂;  偶氮苯;  牛血清白蛋白;  光控
Keywords:
photosensitive surfactant;  azobenzene;  bovine serum aibumin;  optically controlled
分类号:
O 648.11
DOI:
10.3969/j.issn.2095-0411.2016.05.005
文献标志码:
A
摘要:
合成出一种新型光敏性表面活性剂-偶氮苯-4-氧己基三甲基溴化铵(AZO-OHTAB),通过核磁共振谱及质谱分析表征其结构,通过电导率法、表面张力法以及紫外可见分光光谱法探讨其光敏性能,并对比分析365 nm紫外光与可见光下其结构及聚集状态的变化。研究结果表明,365 nm光照射可使AZO-OHTAB发生反式向顺式结构转变,并明显增加其临界胶束浓度(cmc)值。进一步探索其与牛血清白蛋白(BSA)的相互作用,研究表明,反式AZO-OHTAB与BSA相互作用明显强于顺式AZO-OHTAB与BSA的相互作用,通过冷冻蚀刻-透射电镜及流体力学半径的测定可以证明365 nm紫外光对AZO-OHTAB与BSA的相互作用具有光控构象效应。
Abstract:
The novel photosensitive surfactant-azobenzene-4-oxohexyl trimethyl ammonium bromide(AZO-OHTAB)was synthesized. The product was characterized by 1H NMR, LC-MS, conductivity, surface tension and UV visible spectrophotometry, and then compared the change of structure and aggregation state in UV light at 365 nm and visible light. The results showed that the structure of AZO-OHTAB changed from trans to cis structure and the critical micella concentration(cmc)values significantly increased at the 365 nm light irradiation. The interaction mechanism between AZO-OHTAB with bovine serum albumin(BSA)was investigated by freeze etching-TEM and the measurement of hydrodynamic radius. Results showed that the interaction between the trans AZO-OHTAB with BSA was stronger than the cis AZO-OHTAB with BSA, and the UV light at 365 nm could control the conformational change in the interaction of BSA and AZO-OHTAB.

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备注/Memo

备注/Memo:
收稿日期:2016-03-30。基金项目:江苏省自然科学青年基金资助项目( BK20130247)。作者简介:刘文杰(1980—),男,江苏兴化人,博士,讲师,主要从事胶体与界面化学研究。通讯联系人:姚超(1969—),E-mail:yaochao420@163.com
更新日期/Last Update: 2016-10-10