[1]蒋 媛,张 倩,吴雨丹,等.基质金属蛋白酶活性在3种肺疾病中的比较[J].常州大学学报(自然科学版),2017,(02):47-51.[doi:10.3969/j.issn.2095-0411.2017.02.009]
　JIANG Yuan,ZHANG Qian,WU Yudan,et al.Comparison of Matrix Metalloproteinase Activity Between Three Lung Diseases[J].Journal of Changzhou University(Natural Science Edition),2017,(02):47-51.[doi:10.3969/j.issn.2095-0411.2017.02.009]
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基质金属蛋白酶活性在3种肺疾病中的比较()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2017年02期
页码:
47-51
栏目:
生物医学工程
出版日期:
2017-03-28

文章信息/Info

Title:
Comparison of Matrix Metalloproteinase Activity Between Three Lung Diseases
作者:
蒋 媛1; 张 倩2; 吴雨丹1; 周晓鹰1
1. 常州大学 制药与生命科学学院,江苏 常州 213164; 2. 常州市第二人民医院 呼吸科,江苏 常州 213164
Author(s):
JIANG Yuan1;  ZHANG Qian2;  WU Yudan1;  ZHOU Xiaoying1
1. School of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou 213164, China; 2. Department of Respiratory, Second People’s Hospital of Changzhou City, Changzhou 213164, China
关键词:
基质金属蛋白酶;  哮喘;  慢性阻塞性肺病;  肺癌
Keywords:
matrix metalloproteinase(MMP);  asthma;  chronic obstructive pulmonary disease;  lung carcinoma
分类号:
R 392.7
DOI:
10.3969/j.issn.2095-0411.2017.02.009
文献标志码:
A
摘要:
探究基质金属蛋白酶(MMPs)在慢性阻塞性肺病(COPD)、哮喘和肺癌患者中的表达和活性的差异。采用明胶酶谱法来检测COPD、哮喘和肺癌患者血清中MMP表达和活性,其中作图和数据分析都是采用GraphPad Prism software。结果发现,COPD患者表达MMP-9的水平与哮喘患者相比较高(P<0.05),比肺癌患者相比表达水平更高(P<0.000 1),但是MMP-2在3种病人中表达无明显差异。COPD和哮喘患者表达Pro-MMP-9的概率大于肺癌患者。在COPD、哮喘和肺癌患者中,MMPs酶活性表达存在差异。
Abstract:
To explore the serum levels of matrix metalloproteinase(MMPs)in the patients with chronic obstructive pulmonary disease(COPD), asthma and lung carcinoma were compared. Gelatin zymography was used to detect the gelatinolytic activity in serum of the patients with COPD, asthma and lung carcinoma; GraphPad Prism software was used for photography and statistic analysis. The serum levels of MMP-9 in the patients with COPD were significantly higher than asthmatics(P<0.05)and lung carcinoma(P<0.000 1). However, there were no significant differences observed among these three patient’s groups in the expression level of MMP-2 in serums. The probability of Pro-MMP-9 expression in the patients with COPD and asthma appeared more frequently than the patients with lung carcinoma. There are significant serum level differences of MMP-9 expression between patients with COPD, asthma and lung carcinoma.

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备注/Memo

备注/Memo:
收稿日期:2016-05-28。
基金项目:常州科技局研究基金(KYJ1520305); 常州大学高层次人才引进基金(ZMF14020066)。
作者简介:蒋媛(1992—),女,江苏镇江人,硕士生。通讯联系人: 周晓鹰(1957—),E-mail:xiaoyingzhou@cczu.edu.cn
更新日期/Last Update: 2017-04-01