[1]李凌璞,张建莹,林 珊,等.QuEChERS/气相色谱-质谱/质谱法测定蔬菜中58种高风险农药残留量[J].常州大学学报(自然科学版),2019,31(03):40-48.[doi:10.3969/j.issn.2095-0411.2019.03.004]
　LI Lingpu,ZHANG Jianying,LIN Shan,et al.Determination of 58 High Risk Pesticide Residues in Vegetables by QuEChERS Method and Gas Chromatography-Tandem Mass Spectrometry[J].Journal of Changzhou University(Natural Science Edition),2019,31(03):40-48.[doi:10.3969/j.issn.2095-0411.2019.03.004]
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QuEChERS/气相色谱-质谱/质谱法测定蔬菜中58种高风险农药残留量()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
第31卷
期数:
2019年03期
页码:
40-48
栏目:
化学化工
出版日期:
2019-05-28

文章信息/Info

Title:
Determination of 58 High Risk Pesticide Residues in Vegetables by QuEChERS Method and Gas Chromatography-Tandem Mass Spectrometry
文章编号:
2095-0411(2019)03-0040-09
作者:
李凌璞; 张建莹; 林 珊; 赵琼晖; 钟恬恬; 洪旭然
(深圳海关 食品检验检疫技术中心,广东 深圳 518045)
Author(s):
LI Lingpu;  ZHANG Jianying;  LIN Shan;  ZHAO Qionghui;  ZHONG Tiantian;  HONG Xura
(Food Inspection & Quarantine Center, Shenzhen Customs, Shenzhen 518045, China)
关键词:
QuEChERS;  气相色谱-质谱/质谱;  农药残留
Keywords:
QuEChERS;  GC-MS/MS;  pesticide residues
分类号:
O 652.1
DOI:
10.3969/j.issn.2095-0411.2019.03.004
摘要:
优化QuEChERS前处理方法,结合气相色谱-串联四极杆质谱技术,建立了蔬菜中58种禁限用和高风险农药残留测定方法。样品用含体积分数为0.1%乙酸的乙腈溶液提取,经基质分散固相萃取净化,气相色谱-质谱/质谱法测定。结果表明:在线性范围0.010～0.200 mg/L内,58种农药的线性相关系数(r2)均大于0.99,定量限均为0.010 mg/kg。分析物添加水平在0.010,0.050 mg/kg和0.100 mg/kg时,回收率范围在71.2%～120%,相对标准偏差(RSD)为1.68%～14.8%。方法快速简便、灵敏度高,重现性和稳定性好,适用于日常蔬菜中农药多残留的检测。
Abstract:
A method for the determination of 58 banned, restricted and high risk pesticide residues in vegetables was developed by gas chromatography-tandem quadrupole mass spectrometry(GC-MS/MS)with modified QuEChERS pretreatment method. The sample was extracted with acetonitrile containing 0.1% acetic acid, cleaned-up with dispersive solid phase extraction, determined by GC-MS/MS. The results showed that the calibration curves of 58 pesticides were linear in the range of 0.010—0.200 mg/L with correlation coefficients(r2)higher than 0.99. The limits of quantitations for the method were 0.010 mg/kg. The recoveries were 71.2%—120% with added concentration as 0.010,0.050 mg/kg and 0.100 mg/kg, the RSD of the method were between 1.68% and 14.8%. The method showed the advantages of quickness, simplicity, high sensitivity, good reproducibility and stability, and was suitable for the analysis of pesticide residues in daily vegetables.2

参考文献/References:

[1]简秋,朱光艳,郑尊涛,等.国际禁限用农药残留限量标准制定原则简析[J]. 农药科学与管理,2014,35(6):20-25.
[2]任科宇,张文林,曹淑瑞,等.基于柑橘皮渣纳米多孔碳分散固相萃取-气相色谱法测定果蔬中14种有机磷农药残留[J]. 分析化学,2017,45(11):1669-1677.
[3]刘潇威,买光熙,李凌云,等. 蔬菜和水果中有机磷、有机氯、拟除虫菊酯和氨基甲酸酯类农药多残留的测定:NY/T 761—2008[S].北京:中国农业出版社,2008.
[4]农业部,国家食品药品监督管理总局.水果和蔬菜中500种农药及相关化学品残留量的测定 气相色谱-质谱法:GB 23200.8—2016[S].北京:中国标准出版社,2017.
[5]曲栗,李优,曾静,等.气相色谱-三重四极杆质谱法测定植物源性食品中20种拟除虫菊酯类农药残留[J].色谱,2017,35(7):778-784.
[6]张建莹,邓慧芬,李月梅,等.QuEChERS/超高效液相色谱-串联质谱法测定果蔬中122种香港规例农药残留分析[J].测试学报,2017,36(7):849-857.
[7]秦富,邓全道,李湧,等.液相色谱-串联质谱法测定月柿中187种农药残留[J].分析测试学报,2018,37(8):879-886.
[8]鹿剑,任水英.QuEChERS方法在食品中多组分农药残留检测中的研究进展[J].中国酿造,2016,35(9):28-32.
[9]农业农村部,国家市场监督管理局,国家卫生健康委员会.植物源性食品中208种农药及其代谢物残留量的测定 气相色谱-质谱联用法:GB 23200.113—2018[S].北京:中国农业出版社,2018.
[10]吴卫东,洪小柳,孙娅楠,等.GC-MS/MS法快速检测香港《食物内除害剂残余规例》中的101种除害剂残留量[J].环境化学,2016,35(10):2215-2220.
[11]HE Z Y, WANG Y H, WANG L, et al. Determination of 255 pesticides in edible vegetable oils using QuEChERS method and gas chromatography tandenm mass spectrometry[J]. Analytical and Bioanalytical Chemistry, 2017, 409(4):1017-1030.
[12]CAO X L, LIU S H, YANG X Q, et al. A modified QuEChERS sample preparation method for simultaneous determination of 62 pesticide residues in edible fungi using gas chromatograohy-triple quadrupole mass spectrometry[J]. Food Analytical Methods,2016, 9(1):263-274.
[13]符靖雯,林玉婵,黄子敬,等.QuEChERS 结合UHPLC-MS/MS及GC-MS/MS测定黄秋葵中多种农药残留[J].分析试验室,2017,36(5):576-588.
[14]袁荷芳,徐霞,余花,等.气相色谱-串联质谱法快速测定果蔬中20种有机磷农药残留量[J].理化检测-化学分册,2017,53(8):928-932.
[15]甘静妮,程雪梅,莫晓君,等.分散固相萃取/气相色谱-三重四极杆串联质谱测定蔬菜和水果中67种农药残留[J]. 分析测试学报,2016,35(12):1528-1534.

备注/Memo

备注/Memo:
收稿日期:2018-10-26。
基金项目:国家质检总局资助项目(2017IK271,2017IK262)。
作者简介:李凌璞(1984—),女,湖南衡阳人,硕士,助理研究员。通信联系人:张建莹(1977—),E-mail:zhangjianying1001@163.com
更新日期/Last Update: 2019-05-29