[1]黄晓佳,生程钜,周俊涛,等.含奎宁水凝胶的构建及其抗炎作用[J].常州大学学报(自然科学版),2020,32(05):81-87.[doi:10.3969/j.issn.2095-0411.2020.05.011]
　HUANG Xiaojia,SHENG Chengju,ZHOU Juntao,et al.Preparation of Hydrogel with Quinine and Its Anti-Inflammatory Effects[J].Journal of Changzhou University(Natural Science Edition),2020,32(05):81-87.[doi:10.3969/j.issn.2095-0411.2020.05.011]
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含奎宁水凝胶的构建及其抗炎作用()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
第32卷
期数:
2020年05期
页码:
81-87
栏目:
生物医药工程
出版日期:
2020-09-28

文章信息/Info

Title:
Preparation of Hydrogel with Quinine and Its Anti-Inflammatory Effects
文章编号:
2095-0411(2020)05-0081-07
作者:
黄晓佳; 生程钜; 周俊涛; 周 婷; 周 超
常州大学 生物医学工程与健康科学研究院,江苏 常州 213164
Author(s):
HUANG Xiaojia;  SHENG Chengju;  ZHOU Juntao;  ZHOU Ting;  ZHOU Chao
Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China
关键词:
透明质酸;  海藻酸钠;  奎宁;  水凝胶;  抗炎作用
Keywords:
hyaluoronic acid;  sodium alginate;  quinine;  hydrogels;  anti-inflammatory effects
分类号:
R 318
DOI:
10.3969/j.issn.2095-0411.2020.05.011
文献标志码:
A
摘要:
为提高透明质酸水凝胶的力学性能和释放药物的效果,通过引入海藻酸钠制作了透明质酸/海藻酸钠凝胶,并在其中加入了盐酸奎宁,对水凝胶的微观结构、理化性质和生物学性能进行了评价。红外光谱分析表明透明质酸、海藻酸钠、奎宁进行了良好的混合。SEM照片显示材料表面有均一的结构,内部有空洞。材料的力学性能和内部结构稳定。巨噬细胞细胞存活率实验表明水凝胶浸提液没有明显毒性,且可抑制脂多糖诱导的巨噬细胞活化,减少其释放炎症因子和细胞因子等炎症介质,获得的含奎宁水凝胶为皮肤材料提供了新的选择。
Abstract:
The biomedical applications of hyaluronic acid(HA)/sodium alginate(Alg)based hydrogels have been widely studied, showing the potentials in tissue engineering and wound healing. In this study, the quinine was introduced into the HA/Alg-based hydrogel to prepare anti-inflammatory hydrogel. Micro-structure, physicochemical properties were investigated. The results showed that the hydrogels possessed a uniform structure with pores inside, exhibited similar mechanical performance with or without quinine. Employing the in vitro model of lipopolysaccharides(LPS)-induced inflammation, the effects of the hydrogels on inflammation in cultured RAW 264.7 mouse macrophages was determined. The results showed that all the hydrogels did not affect the cell availability. In addition, the LPS-induced interleukin-1(IL-1), IL-6, tumor necrosis factor-α production was inhibited by the conditional medium of the hydrogels with quinine at 20-80 mg/mL, indicating its significant anti-inflammatory activity. Taken together, the prepared hydrogels with quinine may be an anti-inflammatory biomaterials and have potential application in skin wound healing.

参考文献/References:

[1]DE OLIVEIRA S, ROSOWSKI E E, HUTTENLOCHER A. Neutrophil migration in infection and wound repair: going forward in reverse[J]. Nat Rev Immunol, 2016, 16: 378-391.
[2]EMING S A, WYNN T A, MARTIN P. Inflammation and metabolism in tissue repair and regeneration[J]. Science, 2017, 356: 1026-1030.
[3]ZHAO X, LANG Q, YILDIRIMER L, et al. Photocrosslinkable gelatin hydrogel for epidermal tissue engineering[J]. Adv Healthc Mater, 2016, 5: 108-118.
[4]YUK H, ZHANG T, PARADA G A, et al. Skin-inspired hydrogel-elastomer hybrids with robust interfaces and functional microstructures[J]. Nat Commun, 2016, 7: 12028.
[5]MEHRALI M, THAKUR A, PENNISI C P, et al. Nanoreinforced hydrogels for tissue engineering: biomaterials that are compatible with load-bearing and electroactive tissues[J]. Adv Mater, 2017, 29: 1603612.
[6]YUK H, LIN S, MA C, et al. Hydraulic hydrogel actuators and robots optically and sonically camouflaged in water[J]. Nat Commun, 2017, 8: 14230.
[7]MURPHY S V, SKARDAL A, SONG L, et al. Solubilized amnion membrane hyaluronic acid hydrogel accelerates full-thickness wound healing[J]. Stem Cells Transl Med, 2017, 6: 2020-2032.
[8]MOSHAYEDI P, NIH L R, LLORENTE I L, et al. Systematic optimization of an engineered hydrogel allows for selective control of human neural stem cell survival and differentiation after transplantation in the stroke brain[J]. Biomaterials, 2016, 105: 145-155.
[9]DESAI R M, KOSHY S T, HILDERBRAND S A, et al. Versatile click alginate hydrogels crosslinked via tetrazine-norbornene chemistry[J]. Biomaterials, 2015, 50: 30-37.
[10]PARK H, LEE H J, AN H, et al. Alginate hydrogels modified with low molecular weight hyaluronate for cartilage regeneration[J]. Carbohydr Polym, 2017, 162: 100-107.
[11]JONES R A, PANDA S S, HALL C D. Quinine conjugates and quinine analogues as potential antimalarial agents[J]. Eur J Med Chem, 2015, 97: 335-355.
[12]DI A, XIONG S, YE Z, et al. The TWIK2 potassium efflux channel in macrophages mediates NLRP3 inflammasome-induced inflammation[J]. Immunity, 2018, 49: 56-65.
[13]KANG K J, MIN B H, LEE J H, et al. Alginate hydrogel as a potential alternative to hyaluronic acid as submucosal injection material[J]. Dig Dis Sci, 2013, 58: 1491-1496.
[14]CHARRIER E E, POGODA K, WELLS R G, et al. Control of cell morphology and differentiation by substrates with independently tunable elasticity and viscous dissipation[J]. Nat Commun, 2018, 9: 449.
[15]COX J N, RAHMAN M A, BAO S, et al. Cadmium attenuates the macrophage response to LPS through inhibition of the NF-kappa B pathway[J]. Am J Physiol Lung Cell Mol Physiol, 2016, 311: L754-L765.
[16]NAYAK T K S, ALAMURU-YELLAPRAGADA N P, PARSA K V L. Deubiquitinase USP12 promotes LPS induced macrophage responses through inhibition of I kappa B alpha[J]. Biochem Biophys Res Commun, 2017, 483: 69-74.

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备注/Memo

备注/Memo:
收稿日期:2020-04-22。
基金项目:江苏省自然科学基金资助项目(BK20180963)。
作者简介:黄晓佳(1980—),男,湖北咸宁人,博士,副教授。E-mail:huangxj@cczu.edu.cn
更新日期/Last Update: 2020-09-20