参考文献/References:
[1] LIN S, QIN Z, CHEN Q M, et al. Efficient immobilization of bacterial GH family 46 chitosanase by carbohydrate-binding module fusion for the controllable preparation of chitooligosaccharides[J]. Journal of Agricultural and Food Chemistry, 2019, 67(24): 6847-6855.
[2] SALAH R, MICHAUD P, MATI F,et al. Anticancer activity of chemically prepared shrimp low molecular weight chitin evaluation with the human monocyte leukaemia cell line, THP-1[J]. International Journal of Biological Macromolecules, 2013, 52: 333-339.
[3] RAHIMNEJAD S, YUAN X L, WANG L, et al. Chitooligosaccharide supplementation in low-fish meal diets for Pacific white shrimp(Litopenaeus vannamei): effects on growth, innate immunity, gut histology, and immune-related genes expression[J]. Fish & Shellfish Immunology, 2018, 80: 405-415.
[4] MA C R, LI X, YANG K, et al. Characterization of a new chitosanase from a marine bacillus sp. and the anti-oxidant activity of its hydrolysate[J]. Marine Drugs, 2020, 18(2): E126.
[5] PARK P J, JE J Y, KIM S K. Free radical scavenging activity of chitooligosaccharides by electron spin resonance spectrometry[J]. Journal of Agricultural and Food Chemistry, 2003, 51(16): 4624-4627.
[6] YUE L, LI J R, CHEN W W, et al. Geraniol grafted chitosan oligosaccharide as a potential antibacterial agent[J]. Carbohydrate Polymers, 2017, 176: 356-364.
[7] PECHSRICHUANG P, LORENTZEN S B, AAM B B, et al. Bioconversion of chitosan into chito-oligosaccharides(CHOS)using family 46 chitosanase from Bacillus subtilis(BsCsn46A)[J]. Carbohydrate Polymers, 2018, 186: 420-428.
[8] ZHANG J P, CAO H L, LI S G, et al. Characterization of a new family 75 chitosanase from Aspergillus sp. W-2[J]. International Journal of Biological Macromolecules, 2015, 81: 362-369.
[9] YORINAGA Y, KUMASAKA T, YAMAMOTO M, et al. Crystal structure of a family 80 chitosanase from Mitsuaria chitosanitabida[J]. FEBS Letters, 2017, 591(3): 540-547.
[10] ZHOU Z P, ZHAO S Z, LIU Y, et al. A highly conserved aspartic acid residue of the chitosanase from bacillus sp. TS is involved in the substrate binding[J]. Applied Biochemistry and Biotechnology, 2016, 180(6): 1167-1179.
[11] TAKASUKA T E, BIANCHETTI C M, TOBIMATSU Y, et al. Structure-guided analysis of catalytic specificity of the abundantly secreted chitosanase SACTE_5457 from Streptomyces sp. SirexAA-E[J]. Proteins, 2014, 82(7): 1245-1257.
[12] LYU Q Q, SHI Y H, WANG S, et al. Structural and biochemical insights into the degradation mechanism of chitosan by chitosanase OU01[J]. Biochimica et Biophysica Acta, 2015, 1850(9): 1953-1961.
[13] GUO J, RAO Z M, YANG T W, et al. Enhancement of the thermostability of Streptomyces kathirae SC-1 tyrosinase by rational design and empirical mutation[J]. Enzyme and Microbial Technology, 2015, 77: 54-60.
[14] ZHANG S B, WU Z L. Identification of amino acid residues responsible for increased thermostability of feruloyl esterase A from Aspergillus Niger using the PoPMuSiC algorithm[J]. Bioresource Technology, 2011, 102(2): 2093-2096.
[15] WANG X, HAN S Q, YANG Z J, et al. Improvement of the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by engineering C-terminal amino acids[J]. Journal of Biotechnology, 2015, 212: 92-98.
[16] GUO J, WANG Y, GAO W J, et al. Gene cloning, functional expression, and characterization of a novel GH46 chitosanase from streptomyces avermitilis(SaCsn46A)[J]. Applied Biochemistry and Biotechnology, 2022, 194(2): 813-826.
[17] TROTT O, OLSON A J. AutoDock vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading[J]. Journal of Computational Chemistry, 2010, 31(2): 455-461.
[18] PHILLIPS J C, BRAUN R, WANG W, et al. Scalable molecular dynamics with NAMD[J]. Journal of Computational Chemistry, 2005, 26(16): 1781-1802.
[19] GU Z H, LAI J L, HANG J H, et al. Theoretical and experimental studies on the conformational changes of organic solvent-stable protease from Bacillus sphaericus DS11 in methanol/water mixtures[J]. International Journal of Biological Macromolecules, 2019, 128: 603-609.
[20] SUN F D, WANG H P, LIU Q, et al. Effects of temperature and pH on the structure of a protease from Lactobacillus brevis R4 isolated from Harbin dry sausage and molecular docking of the protease to the meat proteins[J]. Food Bioscience, 2021, 42: 101099.
[21] LIU B H, ZHANG J, FANG Z, et al. Enhanced thermostability of keratinase by computational design and empirical mutation[J]. Journal of Industrial Microbiology & Biotechnology, 2013, 40(7): 697-704.
[22] VOGT G, ARGOS P. Protein thermal stability: hydrogen bonds or internal packing?[J]. Folding and Design, 1997, 2: S40-S46.
(责任编辑:李艳,周安迪)