[1]陈鹰娜,李秀文,周晓鹰.孟河古方龙火汤治疗类风湿关节炎的机制[J].常州大学学报(自然科学版),2022,34(06):84-92.[doi:10.3969/j.issn.2095-0411.2022.06.010]
 CHEN Yingna,LI Xiuwen,ZHOU Xiaoying.Mechanism of Classic Menghe Receipt Longhuotang in Treating Rheumatoid Arthritis[J].Journal of Changzhou University(Natural Science Edition),2022,34(06):84-92.[doi:10.3969/j.issn.2095-0411.2022.06.010]
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孟河古方龙火汤治疗类风湿关节炎的机制()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
第34卷
期数:
2022年06期
页码:
84-92
栏目:
生物医药工程
出版日期:
2022-11-28

文章信息/Info

Title:
Mechanism of Classic Menghe Receipt Longhuotang in Treating Rheumatoid Arthritis
文章编号:
2095-0411(2022)06-0084-09
作者:
陈鹰娜李秀文周晓鹰
(常州大学药学院,江苏常州213164)
Author(s):
CHEN Yingna LI Xiuwen ZHOU Xiaoying
(School of Pharmacy, Changzhou University, Changzhou 213164, China)
关键词:
龙火汤 类风湿关节炎 滑膜成纤维细胞 胶原诱导关节炎大鼠 炎症因子
Keywords:
Longhuotang rheumatoid arthritis synovial fibroblasts collagen induced arthritis rat inflammatory factor
分类号:
R 285.5
DOI:
10.3969/j.issn.2095-0411.2022.06.010
文献标志码:
A
摘要:
体内实验建立胶原诱导关节炎(CIA)大鼠模型,记录大鼠体质量、爪厚、关节炎评分,通过明胶酶谱法、酶联免疫吸附实验法探究龙火汤(LHT)对CIA大鼠的作用机制; 体外实验提取新鲜滑膜成纤维细胞(FLS),通过细胞活力实验、划痕实验、明胶酶谱法观察LHT对FLS增殖和迁移的影响。体内实验表明,LHT可以减轻CIA大鼠的关节肿胀,减少关节内的炎性浸润,同时降低血液中基质金属蛋白酶(MMPs)及炎症因子的表达; 体外实验表明,LHT抑制过度激活的FLS增殖和迁移。综上所述,研究通过动物体内实验和体外细胞实验初步证实LHT可以通过降低血液炎症因子和抑制FLS过度增殖来有效缓解类风湿关节炎症状,并初步解释其药理学机制。
Abstract:
The vivo experiment of this study first established a collagen induced arthritis(CIA)rat model, and explored the mechanism of Longhuotang(LHT)on CIA rats by recording the body weight, paw thickness, arthritis score, gelatin Zymogram and enzyme-linked immunosorbent assay. In vitro experiments, fresh synovial fibroblasts(FLS)were first extracted, and the effects of LHT on FLS proliferation and migration were observed by cell viability test, scratch test and gelatin zymogram. The vivo experiments showed that LHT could reduce the joint swelling, the inflammatory infiltration in the joints, and the expression of MMPs and inflammatory factors in the blood of CIA rats. The vitro experiments showed that LHT could inhibited the proliferation and migration of hyperactivated FLS. To sum up, this study preliminarily confirmed that LHT could effectively alleviate rheumatoid arthritis symptoms by reducing blood inflammatory factors and inhibiting FLS excessive proliferation through animal in vivo experiments and in vitro cell experiments, and preliminarily explained its pharmacological mechanism.

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(责任编辑:李艳,周安迪)

备注/Memo

备注/Memo:
收稿日期: 2022-05-16。
基金项目: 常州大学高层次人才引进资助项目(ZMF14020066); 常州大学大学生创新创业资助项目(2022-B-19)。
作者简介: 陈鹰娜(1982—), 女, 江苏常州人, 博士, 讲师。通信联系人: 周晓鹰(1957—), E-mail: xiaoyingzhou@cczu.edu.cn
更新日期/Last Update: 1900-01-01