[1]盛　梅,朱海鸥,戚　俊.脂肪酶催化酯化反应动力学[J].常州大学学报(自然科学版),2000,(03):27-29.
　SHENG Mei,ZHU Hai -ou,QI Jun.Kinetics Studies on Lipase-Catalyzed Esterification of Oleic Acid and Dodecanol in Hexane[J].Journal of Changzhou University(Natural Science Edition),2000,(03):27-29.
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脂肪酶催化酯化反应动力学()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2000年03期
页码:
27-29
栏目:
出版日期:
2000-09-25

文章信息/Info

Title:
Kinetics Studies on Lipase-Catalyzed Esterification of Oleic Acid and Dodecanol in Hexane
作者:
盛　梅1 ;  朱海鸥2 ;  戚　俊2
1.江苏石油化工学院化学工程系, 江苏 常州 213016
Author(s):
SHENG Mei1 ;  ZHU Hai -ou2 ;  QI Jun2
1 .Department of Chemical Engineering , Jiangsu Institute of Petrochemical Technology , Changzhou 213016 , China
关键词:
酯化; 脂肪酶; 动力学
Keywords:
esterification ; lipase ; kinetics
分类号:
O 643.32;Q 556
文献标志码:
A
摘要:
在正己烷中应用工业用脂肪酶催化油酸和正十二醇的酯合成反应, 研究了该反应的动力学机制, 考察了酶和底物浓度对 反应初速度的影响。结果发现:反应过程中不存在底物抑制及扩散限制, 反应为动力学控制, 符合Mi chaelic-Menton 方程。脂 肪酶催化动力学符合Ping -Pong Bi Bi 机理。
Abstract:
The kinetics of lipase -catalyzed esterification of oleic acid and dodecanol in hexane was investigated .The effect of enzyme and substrate concentration on the initial rate of esterification was studied , and the reaction kinetics was described in terms of the Michaelis -Menton mechanism .No evidence of inhibition by oleic acid and dodecanol was found at all substrate concentrations .The kinetics constants were measured for the reaction in the absence of any significant diffusional limitations .It was suggested that the kinetics of the enzymatic reaction agreed with a Ping -Pong Bi Bi mechanism .

参考文献/References:

[1] Yee P Y, Bushra A D.Kinetics Studies on Immobilized Lipase Esterification of Oleic Acid and Octanol [J] .J Chem Tech Biotechnol , 1996 , 65 :239 -248.
[2] Macrae A R.Tailored Triacylglycerols and Esters [J] .Biochem Soc Trans , 1989 , 17 (6):1 146 -1 148 .
[3] Posorke L H , Lefebvre G K, Miller C A , et al.Process Considerations of Continuous Fat Modifi cation with an Immobilized Lipase [J] .J Am Oil Chem Soc, 1988 , 65 (6):922 -930 .
[4] Ramamurthi S , Bhi rud P R, McCurdy A R.Enzymatic Methylation of Canola Oil Deodorizer Distillate [J] .J Am Oil Chem Soc , 1991 , 68 (12):970-975 .
[5] Ramamurthi S , McCurdy A R.Lipase-catalyzed Esterification of Oleic Acid and Methanol in Hexane A Kinetic Study [J] .J Am Oil Chem Soc, 1994 , 71 (9):927-930 .
[6] 曹国民, 黄杰, 高广达.蚕丝固定化脂肪酶的研究[J] .生物 工程学报, 1997 , 13 (1):88-92 .
[7] 曹国民, 盛梅, 高广达.蚕丝固定化脂肪酶催化性能研究[J] .日用化学工业, 1997 , 6 :11 -13 .
[8] Puri ch D L.In Contemporary Enzyme Kineti cs and Mechanism [M] . New York :Academic Press , 1983.254.

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备注/Memo

备注/Memo:
作者简介:盛梅(1964-), 女, 江苏江阴人, 副教授, 硕士;2 -本院化学工程系99 届毕业生。
更新日期/Last Update: 2000-09-25