[1]蔡志强,韩金多,李　亮,等.茶多酚与DNA 相互作用的初步探讨[J].常州大学学报(自然科学版),2002,(03):25-27.
　CAI Zhi -qiang,HAN Jin -duo,LI Liang,et al.Preliminary Studies on Interaction of TP and DNA[J].Journal of Changzhou University(Natural Science Edition),2002,(03):25-27.
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茶多酚与DNA 相互作用的初步探讨()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2002年03期
页码:
25-27
栏目:
出版日期:
2002-09-25

文章信息/Info

Title:
Preliminary Studies on Interaction of TP and DNA
作者:
蔡志强1 ;  韩金多2 ;  李　亮1 ;  李尔炀1 ;  吴小伦3
1.江苏石油化工学院生物工程实验室, 江苏 常州 213016 ;2.上饶农校, 江西上饶334000;3.珠海市出入境检验检疫 局, 广东珠海519015
Author(s):
CAI Zhi -qiang1 ;  HAN Jin -duo2 ;  LI Liang1 ;  LI Er -yang1 ;  WU Xiao -lun3
1 .Biochemistry Engineering Research Lab ., Jiangsu Institute of Petrochemical Technology , Changzhou 213016 , China ;2 .Shangrao Ag ricultural Technical Secondary School , Shang rao 334000 , China ;3 .Zhuhai Ent ry -Exit Inspection and Quarantine Bureau
关键词:
茶多酚; 脱氧核糖核酸; 荧光探针; 溴化乙锭
Keywords:
Tea Polyphenols ; DNA ; fluorescence probe ; Ethidium Bromide
分类号:
Q 615
文献标志码:
A
摘要:
利用荧光探针溴化乙锭(EB) 研究了茶多酚与DNA 相互作用时其荧光光谱的变化。实验表明, 在实验质量浓度范围内, 茶多酚对EB -DNA 体系的荧光强度的抑制率几乎成线性关系, 随它们质量浓度的增加, 抑制率也升高, 其半抑制质量浓度为 0.5 mg/ m L;茶多酚的荧光抑制率随质量浓度的变化不存在饱和性, 且抑制率可达95 %以上。并对茶多酚与DNA 分子相互作 用的机理作了初步探讨。
Abstract:
The changes of the fluorescent spectrum were studied by using Fluorescence Probe EB when TP and DNA interacted .The results show ed :the inhibition rate of relative fluorescence intensi ty would rise when the concent ration of TP was elevated , and their correlation was linear .The inhibition rate of relative f luo rescence intensity at 1.25 mg/mL concent ration was 0.902 for TP and the concentration of TP was approximately 0.5 mg/mL when the inhibition rate of relative f luo rescence intensity was 50 percent .The inhibition rate of fluorescence intensity , which was treated with TP , was not saturated w hen the concentration varied , even above 95 percent .Meanw hile , the mechanism of interaction of TP and DNA had been discussed preliminarily .

参考文献/References:

[1] Cao En -hua , Liu Xiao -Qi , Wang Ju -Jun , et al.Ef fect of Natural Antioxidant Tanshinone Ⅱ -A on DNA Damage by Lipid Peroxidation in Liver Cells [J] . Free Radical Biology & Medicine , 1996 , 20 (6):801 -806 .
[2] 赵宝路.氧自由基和天然抗氧化剂[M] .北京:科学出版 社, 1999.286 -300 .
[3] David S Sigman .Chemical Nucleases [J] .Biochemist ry , 1990 , 29 :9 097 -9 105 .

备注/Memo

备注/Memo:
基金项目:江苏石油化工学院科技基金资助 作者简介:蔡志强(1975 -), 男, 江苏新沂人, 硕士, 主要从事生物化学与免疫分析方面的研究。
更新日期/Last Update: 2002-09-25