[1]孙贤祥,邱岳进,谢爱娟,等.合成受体- 盐酸普萘洛尔分子印迹聚合物的 合成与分子识别[J].常州大学学报(自然科学版),2008,(03):50-55.
　SU N Xian- x iang,Q IU Yue- jin,XIE Ai- juan,et al.Synthetic Receptor for Propranolol- Synthesis and Molecular Recognition of Molecularly Imprinted Polymer[J].Journal of Changzhou University(Natural Science Edition),2008,(03):50-55.
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合成受体- 盐酸普萘洛尔分子印迹聚合物的 合成与分子识别()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2008年03期
页码:
50-55
栏目:
出版日期:
2008-06-25

文章信息/Info

Title:
Synthetic Receptor for Propranolol- Synthesis and Molecular Recognition of Molecularly Imprinted Polymer
作者:
孙贤祥1;  邱岳进2 ;  谢爱娟 1 ;  王志勇 3 ;  苏增权3
1.江苏工业学院 化学化工学院 , 江苏 常 州 213 164; 2. 常 州出入境 检验检 疫局, 江苏 常州 213 022; 3. 常州市 天汁化 学 有限公司 , 江苏 常州 21 301 6)
Author(s):
SU N Xian- x iang1 ;  Q IU Yue- jin 2;  XIE Ai- juan1 ;  WAN G Zhi- y ong3;  SU Z eng- quan 3
1. School of Chem istry and Chemical Eng ineering , Jiangsu Poly technic U niver sity, Chang zhou 213 164, China; 2. Changzhou Bur eau of Commo dity Inspectio n & Q uarantine on Entering and Ex iting, Changzho u 213022; 3. T ian Ze Chemical Limited Com pa
关键词:
分子印迹聚合物;  盐酸普萘洛尔;  分子识别;  印迹条件
Keywords:
mo lecularly imprinted po lym er;  pr opranolo l hydrochlor ide;  mo lecule recog nition;  imprinting condition
分类号:
Q 632; R 971
文献标志码:
A
摘要:
以盐酸普萘洛尔为模板分子、 甲基丙烯酸甲酯为单 体、 TM P 为 交联剂, 通 过对分子 印迹聚合 物合成条 件的优选, 合 成 出了 对盐酸普萘洛尔呈高选择识别的分子印迹聚合物。 首次采用有效分配系数 Kc , 并 用 Scatchard 分析等评 价了 分子印迹聚 合 物对底物的吸附特性。 Scatch ard 分析结果表明 , 普萘洛尔- M IP 存在两种分子识 别位点, 其离解常 数 K d1, K d2 和最大表观 结 合量 Q max 1, Q max2 分别为 11 37 @ 10- 5 mol/ L, 91 50 @ 10- 5 mol/ L 和 251 72 Lmol/ g, 691 06 Lmol/ g。 还调查了 普萘洛尔- M IP 对 分子结构部分相似的药物萘普生、 噻吗洛尔等的分子识别行为。
Abstract:
A synthetic receptor for Propranolol hydrochloride was prepared by a non- covalently im printed technique, using Propranolol hy drochloride as a template, methyl methacrylate ( MM A) as a functional monomer and tri- [hydr oxy methy l] - pro pan- tri- acry lic acid ( T M P) as a cro ss- linker, and m ethanol as a po rogenic so lvent. After extraction of tem plate, affinity of the polymer imprinted with Propranolo l hy dro chloride was examined in re- binding experiment. At the first time, an efficient partition coefficient ( Kc ) was adopted to evaluate re- binding property of the imprinted polymer. From the Scatchard analysis, binding site heterog eneity is obvious and two types of binding sites can be identified. The equilibrium dissociation co nstant K d1 and the apparent m ax imum amount Q max1 of the higher affinity binding sites can be calculated to be 11 37 @ 10- 5 mo l/ L, 25.72 Lmo l/ g; while K d2 and Qm ax2, of the lower affinity binding sites w ere calculated to be 91 50 @ 10- 5 mol/ L, 69.06 Lmo l/ g, respectively. M oreover, good mo lecule recogn-i tion behavio r of the imprinted po lymer toward some drugs was observed experim entally.

参考文献/References:

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[10] H au pt K, Dz goev A , M osbach K. A ssay sys tem for h erb-i cide 2, 4 dichlorophenoxyacetic acid using a m olecu larly imprinted polym er as an artificial recogn iti on elemen t [J] . A nal Chem, 1998, 70: 628- 631.

相似文献/References:

[1]董　艳,邱岳进,孙贤祥.盐酸氢氯噻嗪分子印迹聚合物的实验研究[J].常州大学学报(自然科学版),2007,(04):21.
　DONG Yan,QIU Yue -jin,SUN Xian -xiang.Experimental Investigation on the Molecularly Imprinted Polymer Receptor for Hydrochlorothiazide[J].Journal of Changzhou University(Natural Science Edition),2007,(03):21.

备注/Memo

备注/Memo:
作者简介: 孙贤祥 ( 1946- ) , 男 , 江苏句容市人, 教授。
更新日期/Last Update: 2008-09-25