[1]谢爱娟,周 民,罗士平,等.基于聚邻苯二胺/石墨烯修饰电极的肠道病毒71型(EV71)电化学免疫传感器[J].常州大学学报(自然科学版),2013,(01):71-75.[doi:10.3969/j.issn.2095-0411.2013.01.015]
　XIE Ai-juan,ZHOU Min,LUO Shi-ping,et al.Electrochemical Immunosensor for Detection of Human Enterovirus 71 Based on Graphene-Poly(o-Phenylenediamine)Modified Electrode[J].Journal of Changzhou University(Natural Science Edition),2013,(01):71-75.[doi:10.3969/j.issn.2095-0411.2013.01.015]
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基于聚邻苯二胺/石墨烯修饰电极的肠道病毒71型(EV71)电化学免疫传感器()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2013年01期
页码:
71-75
栏目:
出版日期:
2013-01-01

文章信息/Info

Title:
Electrochemical Immunosensor for Detection of Human Enterovirus 71 Based on Graphene-Poly(o-Phenylenediamine)Modified Electrode
作者:
谢爱娟1; 周 民2; 罗士平1; 尹 俊1; 孔 泳1
1.常州大学 石油化工学院,江苏 常州 213164; 2.南京医科大学附属常州二院 血液科,江苏 常州 213003
Author(s):
XIE Ai-juan1; ZHOU Min2; LUO Shi-ping1; YIN Jun1; KONG Yong1
1.School of Petrochemical Engineering,Changzhou University,Changzhou 213164,China; 2.Department of Hematology,Affiliated Changzhou Second Hospital of Nanjing Medical University,Changzhou 213003,China
关键词:
石墨烯;  聚邻苯二胺;  肠道病毒71型;  电化学免疫传感器
Keywords:
graphene;  poly(o-phenylenediamine);  EV71;  electrochemical immunosensor
分类号:
O 657.1
DOI:
10.3969/j.issn.2095-0411.2013.01.015
文献标志码:
A
摘要:
采用改性的Hummers法制备石墨烯氧化物(GO),并将其滴涂在玻碳电极(GCE)表面。通过电化学还原将GO还原为石墨烯,并进一步采用电化学聚合法在石墨烯表面形成聚邻苯二胺(PoPD)膜,从而制备了PoPD/石墨烯修饰GCE。将EV71抗体固定在修饰电极表面,制备新型的电化学免疫传感器。当发生免疫反应时,由于EV71抗原与抗体生成的免疫复合物阻碍了电子的传递,PoPD的氧化峰电流下降。当抗原的浓度在0.1-80 ng/mL范围内,PoPD氧化峰电流的降低值与抗原的浓度成正比。该免疫传感器对EV71的检测限为0.08 ng/mL(信噪比为3)。
Abstract:
Graphene oxide(GO)was prepared by a modified Hummers method and dropped onto the surface of a glassy carbon electrode(GCE). The GO waselectrochemically reduced to graphene, and poly(o-phenylenediamine)(PoPD)film was then electrosynthesized on the surface of graphene to prepare the PoPD/graphene modified GCE. A novel electrochemical immunosensor was obtainedby immobilizing anti-EV71 onto the surface of this modified electrode. When theimmunoreaction between anti-EV71 and EV71 antigen in the solution took place, the electron transfer would be hindered by the produced immunocomplex and thus the oxidation peak current of PoPD would decrease. The decrease in the oxidation peak current of PoPD was proportional to the concentration of EV71 antigen in the range from 0.1 to 80 ng/mL, and the detection limit was as lowas 0.08 ng/mL(signal-to-noise ratio of 3).

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目资助(21275023); 江苏省自然科学基金项目资助(BK2012593) 作者简介:谢爱娟(1968-),女,江苏常州人,实验师; 通讯联系人:孔泳。
更新日期/Last Update: 2013-01-01