[1]蔡志强,荣艳,陈杰,等.新烟碱类杀虫剂哌虫啶降解菌P4-7的筛选、鉴定及其降解特性的研究[J].常州大学学报(自然科学版),2016,(04):87-92.[doi:10.3969/j.issn.2095-0411.2016.04.016]
　CAI Zhiqiang,RONG Yan,CHEN Jie,et al.Isolation, Identification and Degradation Characteristics of a Novel cis-Nitromethylene Neonicotinoid Insecticide Paichongding-Degrading Strain P4-7[J].Journal of Changzhou University(Natural Science Edition),2016,(04):87-92.[doi:10.3969/j.issn.2095-0411.2016.04.016]
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新烟碱类杀虫剂哌虫啶降解菌P4-7的筛选、鉴定及其降解特性的研究()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2016年04期
页码:
87-92
栏目:
出版日期:
2016-07-30

文章信息/Info

Title:
Isolation, Identification and Degradation Characteristics of a Novel cis-Nitromethylene Neonicotinoid Insecticide Paichongding-Degrading Strain P4-7
作者:
蔡志强; 荣艳; 陈杰; 李珊珊; 王静
常州大学 制药与生命科学学院,江苏 常州 213164
Author(s):
CAI Zhiqiang;  RONG Yan;  CHEN Jie;  LI Shanshan;  WANG Jing
School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou 213164, China
关键词:
哌虫啶;  曲霉属;  18S rDNA;  生物降解;  降解途径
Keywords:
Paichongding;  Aspergillus sp.;  18S rDNA;  biodegradation;  degradation pathway
分类号:
X 172
DOI:
10.3969/j.issn.2095-0411.2016.04.016
文献标志码:
A
摘要:
采用富集培养的方法,从常州南郊农田土壤中筛选出8株以哌虫啶(Paichongding, IPP)为唯一生长碳源和能源的菌株,选择生长速度快和降解率较高的菌株P4-7进一步研究,据18S rDNA序列将其鉴定曲霉属(Aspergillus sp.)。通过单因素试验和初步优化,菌株4-7在20 d对50 mg/L哌虫啶的降解率可达30%,该菌株降解哌虫啶的最适初始pH为7.0、最适接种量为5%。菌株Aspergillus sp. P4-7降解IPP的过程中,检测到2 种中间代谢产物M1 和M2,经高分辩LC-MS/MS鉴定为C14H17ClN4O3 (M1)、C14H14ClN3O(M2),实验结果表明,IPP的丙氧基水解成羟其,形成中间产物M1,M1进一步脱硝基,羟基同时氧化形成M2。
Abstract:
Paichongding(IPP)is a novel nitromethylene neonicotinoid recently developed in China. Eight strains which can utilize IPP as sole carbon and energy source were isolated from the field in south of Changzhou, Jiangsu province. The strain P4-7 with higher rate of degrading IPP was selected for further study. Based on 18S rDNA sequence, the strain was identified as Aspergillus sp.. Under the condition of 50 mg/L of IPP, pH 7.0 and 5% of inoculum, the degradation rate of IPP within 20 days reached 30% by the strain P4-7. Two main metabolites(M1 and M2)were detected during IPP degradation process. Based on LC-MS/MS analysis, they were identified as C14H17ClN4O3 (M1)and C14H14ClN3O(M2). According to their appearance dynamics, the biodegradation pathway of IPP by P4-7 was proposed: The propyl group of IPP was initially de-esterified and hydrolyzed to form M1, then M1 was transformed into M2 through reduction of nitro group on M1 and the hydroxyl oxidization.

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备注/Memo

备注/Memo:
收稿日期:2015-12-04。基金项目:国家自然科学基金资助(11275033); 江苏省自然科学基金面上项目(BK20151185)。作者简介:蔡志强(1975—),男, 江苏徐州人,博士,副教授,主要从事环境微生物和现代生物技术等方面的研究。
更新日期/Last Update: 2016-08-30