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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:

期数:

2000年03期

页码:

33-35

栏目:

出版日期:

2000-09-25

文章信息/Info

Title:

A Study on Hydrolysis of Protein in Pearls by Papain

作者:

金凤明¹ ;  李桂贞² ;  孙晓娟¹

1.江苏石油化工学院化学工程系, 江苏 常州 213016;2.华东理工大学分析测试中心, 上海200237

Author(s):

JIN Feng -ming ¹ ;  LI Gui -zheng ² ;  SUN Xiao -juan ¹

1 .Department of Chemical Engineering , Jiangsu Institute of Petrochemical Technology , Changzhou 213016 , China ; 2 .Center of Analysis and Research , East China University of Science and Technology , Shanghai 200237 , China

关键词:

珍珠; 蛋白质; 木瓜酶; 水解

Keywords:

pearls ; protein ; papain ; hydrolysis

分类号:

Q 512.6 ;Q 556.9

文献标志码:

A

摘要:

用异硫氰酸苯酯柱前衍生高效液相色谱法测定了淡水养殖珍珠中角壳蛋白的氨基酸组成, 对影响木瓜酶水解珍珠角壳蛋 白的主要因素如木瓜酶用量、蛋白质质量浓度、反应时间、酸度和温度等进行了研究和优化, 为珍珠资源的有效利用提供依 据。

Abstract:

The contents of amino acids in protein of pearls were determined by reverse -phase high -performance liquid chromatography after precolumn derivatization with phenylisothiocyanate .The effect of such factors as dosage of papain , concentration of protein , time of reaction , acidity , temperature on hydrolytic ratio of protein in pearls by papain was studied .A simple and no -pollution technique for hydrolyzing protein in pearls by papain to obtain amino acids and peptides was developed in order to provide a basis for the effective use of pearl resources in our country

备注/Memo

备注/Memo:

作者简介:金凤明(1962-), 男, 江苏昆山人, 讲师, 硕士, 主要从事分析化学教学及研究工作。

常用功能

更新日期/Last Update: 2000-09-25