[1]傅时波,李尔炀.生物表面活性剂检测方法的研究[J].常州大学学报(自然科学版),2007,(02):23-25.
　FU Shi - bo,L I Er - yang.Study of Method to Detect Biosurfactant[J].Journal of Changzhou University(Natural Science Edition),2007,(02):23-25.
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生物表面活性剂检测方法的研究()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
期数:
2007年02期
页码:
23-25
栏目:
出版日期:
2007-06-25

文章信息/Info

Title:
Study of Method to Detect Biosurfactant
作者:
傅时波;  李尔炀
江苏工业学院化学工程系, 江苏 常州 213164
Author(s):
FU Shi - bo ;  L I Er - yang
Department of Chemist ry Engineering , J iangsu Polytechnic University , Changzhou 213164 , China
关键词:
生物表面活性剂;  检测;  排油圈法;  预测方程
Keywords:
biosurfactant ;  screening ;  oil spreading ;  predicting equation
分类号:
Q 939.1 　
文献标志码:
A
摘要:
生物表面活性剂的检测方法主要有: 液滴坍塌法、排油圈法和血平板法。由于排油圈法具有简单、快速的特点, 在实验 室中常常被应用, 但各批次实验之间由于油膜厚度不同, 导致结果可比性差。研究发现排油圈直径不仅与生物表面活性剂浓度 有关, 还与油膜厚度相关。实验数据利用SAS 810 软件进行二元线性回归, 得到预测模型: Y = - 100105 + 2 391149 X1 + 154119 X2 , R2 = 01968 5 , 得到的预测方程能有效预测表面活性剂浓度的大小, 提高了各批次实验之间结果的可比性。
Abstract:
There are three main methods , drop collapse , oil spreading , and blood agar lysis , to detect biosurfactant production. The oil spreading technique is an easy and quick method. It is frequently applied in the laboratory. However , because the thickness of different test is dissimilar , the results of oil spreading are unable to contrast . The study showed that the curve of the diameter of clear zone was in linear correlation with the concent ration of the biosurfactant and the thickness of oil. All data were processed with software SAS 810. The gained predicting equation was Y = - 100105 + 2 391149 X1 + 154119 X2 , R2 = 0.968 5. The gained predicting equation of regression could predict the concent ration of biosurfactant . The result commeasurability of eachset of experiment was enhanced.

参考文献/References:

[1] 左晶, 王学川1 生物表面活性剂的应用[J] 1 化学工业与工 程技术, 2005 , 26 (2) : 23 - 261
[2] Mulligan C N , Cooper D G, Neufeld R J . Selection of microbes producing biosurfactants in media without hydrocarbons [J] . Fer2 ment Technol , 1984 , 62 : 311 - 3141
[3] Jain D K, Collins - Thompson D L , Lee H , et al. A drop - col2 lapsing test for screening surfactant - producing microorganisms [J] . Microbiol Methods , 1991 , 13 : 271 - 2791
[4] Noha H Y, Kathleen E D , David P N , et al. Comparison of methods to detect biosurfactant production by diverse microorgan2 isms [J] . Microbiol Methods , 2004 , 56 : 339 - 3471
[5] 张凡, 佘跃惠1 排油圈法对生物表面活性剂的定性与定量[J] 1 化学工程师, 2005 , 112 (1) : 14 - 16.
[6] 朱军1 线性模型分析原理[M] 1 第一版1 北京: 科学出版 社, 1999.
[7] 洪楠, 侯军1SAS for windows 统计分析系统教程[M] 1 第一 版1 北京: 电子工业出版社, 2001.

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备注/Memo

备注/Memo:
作者简介: 傅时波(1981 - ) , 男, 浙江温州人, 硕士研究生; 联系人: 李尔炀。
更新日期/Last Update: 2007-06-25