[1]张 宇,周少敏,朱孝霖,等.磁性材料固定化脂肪酶的制备及其催化性能研究[J].常州大学学报(自然科学版),2018,30(04):28-35.[doi:10.3969/j.issn.2095-0411.2018.04.005]
　ZHANG Yu,ZHOU Shaomin,ZHU Xiaolin,et al.Preparation and Catalytical Performance of Immobilized Liphase on Nano-Maganetic Material[J].Journal of Changzhou University(Natural Science Edition),2018,30(04):28-35.[doi:10.3969/j.issn.2095-0411.2018.04.005]
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磁性材料固定化脂肪酶的制备及其催化性能研究()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
第30卷
期数:
2018年04期
页码:
28-35
栏目:
化学化工
出版日期:
2018-07-28

文章信息/Info

Title:
Preparation and Catalytical Performance of Immobilized Liphase on Nano-Maganetic Material
作者:
张 宇; 周少敏; 朱孝霖; 荣 艳; 蔡金燕; 郭 静; 蔡志强
常州大学 制药与生命科学学院,江苏 常州 213164
Author(s):
ZHANG Yu;  ZHOU Shaomin;  ZHU Xiaolin;  RONG Yan;  CAI Jinyan;  GUO Jing;  CAI Zhiqiang
School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou 213164, China
关键词:
固定化;  脂肪酶;  生物柴油;  L-丝氨酸十八醇酯;  生物催化
Keywords:
immobilization;  lipase;  biodiesel;  myristyl serinate;  biocatalysis
分类号:
TQ 925.6
DOI:
10.3969/j.issn.2095-0411.2018.04.005
文献标志码:
A
摘要:
采用新型载体磁性纳米Fe3O4对青霉菌Penicillium sp.Y-29发酵产生的脂肪酶粗酶液固定化,通过电镜和红外分析确定脂肪酶是否成功固定。研究固定化脂肪酶的最优催化条件并催化合成L-丝氨酸十八醇酯。实验发现对于饱和脂肪酸来说,碳个数越多,在合成生物柴油时催化率越高,而对于短链醇,亚甲基个数的增加,酯化率降低。多次加甲醇的方式可有效避免短链醇对酶的变性作用,使酯化率提高。增加底物的量可以促进酯化率,但是过高的醇使酶活性降低,酯化率反而降低。最终获得最优催化条件:硬脂酸、甲醇物质的量比为1:1.7,甲醇分3次加入,5 mL石油醚,5%固定化脂肪酶,160 r/min,30 ℃密闭震荡反应24 h。采用水溶性有机溶剂体积分数为5% DMF时酯化率最高,而随着比例的升高,酯化率明显下降。
Abstract:
Immobilized lipase has more stable catalytic performance with longer life compared to free lipase. The lipase produced by Penicillium sp. Y-29 was immobilized on new carrier- magnetic nanoparticle Fe3O4, and analyzed by SEM and IR. It was successfully immobilized. Catalytic performance of immobilized lipase was investigated including the reaction of different substrate, concentration, the way of adding methanol and its ratio to acid, as well as the reaction life. It was found that for saturated fatty acid, the higher the number of carbon, the higher the catalytic rate in the synthesis of biodised. And the rate was decreased as the number of methylene increased for short chain alcohol. Multiple times adding of methyl alcohol is a good method to prevent denaturation of enzyme activity, and the esterification rate was improved. The increase of substrate promoted the reaction process, but the production was lower when excessive alcohol was added. The attained condition of reaction is: 1:1.7 ratio of stearic acid to methanol, 3 adding times of methanol, 5 mL petroleum ether, 5% immobilized lipase with 160 r/min, 30 ℃ shaking for 24 h. Also, it was to investigate the solvent impact on catalytic performance to produce myristyl serinate. The product was analyzed by HPLC/LC-MS/MRI. When using 5% DMF as the solvent, the production rate was 30.83% with more than 93% purity. The higher percentage of DMF inversely impact the reaction. The study of Penicillium sp.Y-29 immobilized lipase and catalytic performance is significant in the development and industrialization of this lipase.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(11275033); 江苏省自然科学基金面上项目(BK20151185)。
作者简介:张宇(1995—),男,吉林白山人,硕士生。通信联系人:蔡志强(1975 —),E-mail:zhqcai@cczu.edu.cn
更新日期/Last Update: 2018-07-30