[1]洪婷婷,周舒文,崔朋飞,等.毛细管电泳-荧光检测在药物分析中的应用[J].常州大学学报(自然科学版),2021,33(02):68-72.[doi:10.3969/j.issn.2095-0411.2021.02.009]
 HONG Tingting,ZHOU Shuwen,CUI Pengfei,et al.Application of Capillary Electrophoresis-Fluorescence Detection for Pharmaceutical Analysis[J].Journal of Changzhou University(Natural Science Edition),2021,33(02):68-72.[doi:10.3969/j.issn.2095-0411.2021.02.009]
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毛细管电泳-荧光检测在药物分析中的应用()
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常州大学学报(自然科学版)[ISSN:2095-0411/CN:32-1822/N]

卷:
第33卷
期数:
2021年02期
页码:
68-72
栏目:
生物医药工程
出版日期:
2021-03-28

文章信息/Info

Title:
Application of Capillary Electrophoresis-Fluorescence Detection for Pharmaceutical Analysis
文章编号:
2095-0411(2021)02-0068-05
作者:
洪婷婷 周舒文 崔朋飞 邱 琳 蒋鹏举 王建浩
(常州大学 药学院, 江苏 常州 213164)
Author(s):
HONG Tingting ZHOU Shuwen CUI Pengfei QIU Lin JIANG Pengju WANG Jianhao
(School of Pharmacy, Changzhou University, Changzhou 213164, China)
关键词:
毛细管电泳 荧光检测 药物分析
Keywords:
capillary electrophoresis fluorescence detection pharmaceutical analysis
分类号:
R 9
DOI:
10.3969/j.issn.2095-0411.2021.02.009
文献标志码:
A
摘要:
毛细管电泳微分离技术基于其样品用量少, 分离效率高的特点, 在分离分析领域具有其独特的优势。相较于传统紫外检测, 毛细管电泳-荧光检测可实现高灵敏度的分析。围绕药物分析的应用, 着重介绍了毛细管电泳-荧光检测技术用于手性药物的分离、 化学及生物药物的定量分析以及抑制剂的筛选。
Abstract:
Capillary electrophoresis technique presents tremendous advantages in separation science depending on its high efficiency and low sample consumption. Compared with traditional ultraviolet detection, capillary electrophoresis-fluorescence detection can achieve high sensitivity. This review mainly introduces the application of capillary electrophoresis-fluorescence detection for chiral separation, quantification of chemical and biological drugs and screening inhibitors.

参考文献/References:

[1]QUE A H, KONSE T, BAKER A G, et al. Analysis of bile acids and their conjugates by capillary electrochromatography/electrospray ion trap mass spectrometry[J]. Anal Chem, 2000, 72(13): 2703-2710.
[2]LAMMERHOFER M, SVEC F, FRECHET J M J, et al. Capillary electrochromatography in anion-exchange and normal-phase mode using monolithic stationary phases[J]. J Chromatogr A, 2001, 925(1): 265-277.
[3]HARA T, MAKINO S, WATANABE Y, et al. The performance of hybrid monolithic silica capillary columns prepared by changing feed ratios of tetramethoxysilane and methyltrimethoxysilane[J]. J Chromatogr A, 2010, 1217(1): 89-98.
[4]ZHANG Z B, WANG F J, XU B, et al. Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis[J]. J Chromatogr A, 2012, 1256: 136-143.
[5]WIEDER W, LUBBAD S H, TROJER L, et al. Novel monolithic poly(p-methylstyrene-co-bis(p-vinylbenzyl)dimethylsilane)capillary columns for biopolymer separation[J]. J Chromatogr A, 2008, 1191(1): 253-262.
[6]LIU C, DENG Q, FANG G, et al. Ionic liquids monolithic columns for protein separation in capillary electrochromatography[J]. Anal Chim Acta, 2013, 804: 313-320.
[7]WANG T, CHEN Y, MA J, et al. Ionic liquid-based zwitterionic organic polymer monolithic column for capillary hydrophilic interaction chromatography[J]. Analyst, 2015, 140(16): 5585-5592.
[8]ZHANG Z B, WU M H, ZOU H F, et al. Recent progress of chiral monolithic stationary phases in CEC and capillary LC[J]. Electrophoresis, 2010, 31(9): 1457-1466.
[9]WENG X L, BAOZ B, XING H B, et al. Synthesis and characterization of cellulose 3,5-dimethylphenylcarbamate silica hybrid spheres for enantioseparation of chiral-blockers[J]. J Chromatogr A, 2013, 1321: 38-47.
[10]HAMIDI S, JOUYBAN A. Pre-concentration approaches combined with capillary electrophoresis in bioanalysis of chiral cardiovascular drugs[J]. Pharmaceutical Sciences, 2015, 21(4): 229-243.
[11]HERRERO M, IBANEZ E, MARTIN-ALVAREZ P J, et al.Analysis of chiral amino acids in conventional and transgenic maize[J]. Anal Chem, 2007, 79(13): 5071-5077.
[12]LIN K C, HSIEH M M, CHANG C W, et al. Stacking and separation of aspartic acid enantiomers under discontinuous system by capillary electrophoresis with light-emitting diode-induced fluorescence detection[J]. Talanta, 2010, 82(5): 1912-1918.
[13]WAGNER Z, TABI T, JAKO T, et al. Chiral separation and determination of excitatory amino acids in brain samples by CE-LIF using dual cyclodextrin system[J]. Anal Bioanal Chem, 2012, 404(8): 2363-2368.
[14]CREAMER J S, MORAM F, WILLIS P A. Enhanced resolution of chiral amino acids with capillary electrophoresis for biosignature detection in extraterrestrial samples[J]. Anal Chem, 2017, 89(2): 1329-1337.
[15]PRIOR A, COLIVA G, SOMSEN G, et al. Chiral capillary electrophoresis with UV-excited fluorescence detection for the enantioselective analysis of 9-fluorenylmethoxycarbonyl-derivatized amino acids[J]. Anal Bioanal Chem, 2018, 410(20): 4979-4990.
[16]MBUNA J, KANETA T. Capillary electrophoresis with laser-induced fluorescence detection for application in intracellular investigation of anthracyclines and multidrug resistance proteins[J]. Analytical Sciences, 2015, 31(11): 1121-1128.
[17]XIONG G, CHEN Y, ARRIAGA E A. Measuring the doxorubicin content of single nuclei by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection[J]. Anal Chem, 2005, 77(11): 3488-3493.
[18]XIAO Y, LI Y, YING J, et al. Determination of alditols by capillary electrophoresis with indirect laser-induced fluorescence detection[J]. Food Chemistry, 2015, 174: 233-239.
[19]SAAR-REISMAA P, ERME E, VAHER M, et al. In situ determination of illegal drugs in oral fluid by portable capillary electrophoresis with deep UV excited fluorescence detection[J]. Anal Chem, 2018, 90(10): 6253-6258.
[20]CHEN C H, FENG H, GUO R, et al. Intact NIST monoclonal antibody characterization-proteoforms, glycoforms-using CE-MS and CE-LIF[J]. Cogent Chemistry, 2018, 4(1): 1-13.
[21]NGUYEN B T, PARK M, KANG M J. Capillary electrophoresis-laser-induced fluorescence(CE-LIF)-based immunoassay for quantifying antibodies against cyclic citrullinated peptides[J]. Analyst, 2018, 143(13): 3141-3147.
[22]CHEN C, HU Y, LI F, et al. A single-label fluorescent derivatization method for quantitative determination of neurotoxin in vivo by capillary electrophoresis coupled with laser-induced fluorescence detection[J]. Analyst, 2016, 141(14): 4495-4501.
[23]LIN L, LIU S, HUANG Y, et al. Automatic and integrated micro-enzyme assay(AIμEA)platform for highly sensitive thrombin analysis via an engineered fluorescence protein-functionalized monolithic capillary column[J]. Anal Chem, 2015, 87(8): 4552-4559.
[24]GREENOUGH L, SCHERMERHORN K M, GARDNER A F. Adapting capillary gel electrophoresis as a sensitive, high-throughput method to accelerate characterizationof nucleic acid metabolic enzymes[J]. Nucleic Acids Res, 2016, 44(2): 1-11.
[25]XU M, LIU C, KANG J. Screening of small-molecule inhibitors of protein-protein interaction with capillary electrophoresis frontal analysis[J]. Anal Chem, 2016, 88(16): 8050-8057.
[26]JENNIFER N, GESTWICKI J E, KENNEDY R T, et al. Development of a capillary electrophoresis platform for identifying inhibitors of protein-protein interactions[J]. Anal Chem, 2013, 85(20): 9824-9831.

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备注/Memo

备注/Memo:
收稿日期:2020-06-04。
基金项目:国家自然科学基金资助项目(81803495)。
作者简介:洪婷婷(1989—),女,安徽铜陵人,博士,讲师。通信联系人:王建浩(1981—),E-mail: minuswan@cczu.edu.cn
引用本文:洪婷婷, 周舒文, 崔朋飞, 等. 毛细管电泳-荧光检测在药物分析中的应用[J]. 常州大学学报(自然科学版), 2021, 33(2): 68-72.
更新日期/Last Update: 1900-01-01